A new model of experimental hemispherotomy in young adult Rattus norvegicus: a neural tract tracing and SPECT in vivo study

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Matias, Jr., Ivair ^{[1, 2]} ; Elias, Daoud Hibrahim ^[2] ; Bernardino Garcia, Camila Araujo ^[1] ; Silva, Guilherme Henrique ^[1] ; Mejia, Jorge ^[3] ; Cabral, Francisco Romero ^[3] ; Camargo Miranda, Ana Claudia ^[3] ; da Silva, Sergio Gomes ^{[3, 4]} ; Lopes, Luiza da Silva ^[1] ; Coimbra, Norberto Cysne ^{[2, 5]} ; Machado, Helio Rubens ^{[1, 5]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Surg & Anat, Lab Pediat Neurosurg & Dev Neuropathol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, Lab Neuroanat & Neuropsychobiol, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Neuroelectrophysiol Multiuser Ctr, Sao Paulo - Brazil

^[4] Univ Mogi das Cruzes, NPT, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Surg & Anat, Neurobiol Pain & Emot Lab, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF NEUROSURGERY; v. 130, n. 4, p. 1210-1223, APR 2019.
• Citações Web of Science: 1

Resumo

OBJECTIVE The objective of this study was to describe a new experimental model of hemispherotomy performed on laboratory animals. METHODS Twenty-six male young adult Wistar rats were distributed into two groups (surgery and control). The nonfluo-rescent anterograde neurotracer biotinylated dextran amine (BDA; 10,000 MW) was microinjected into the motor cortex area (M1) according to The Rat Brain in Stereotaxic Coordinates atlas to identify pathways and fibers disconnected after the experimental hemispherectomy. SPECT tomographic images of Tc-99m hexamethylpropyleneamine oxime were obtained to verify perfusion in functioning areas of the disconnected and intact brain. A reproducible and validated surgical procedure is described in detail, including exact measurements and anatomical relationships. An additional 30 rodents (n = 10 rats per group) were divided into naive, sham, and hemispherotomy groups and underwent the rotarod test. RESULTS Cortico-cortical neural pathways were identified crossing the midline and contacting neuronal perikarya in the contralateral brain hemisphere in controls, but not in animals undergoing hemispherotomy. There was an absence of perfusion in the left side of the brain of the animals undergoing hemispherotomy. Motor performance was significantly affected by brain injuries, increasing the number of attempts to maintain balance on the moving cylinder in the rotarod test at 10 and 30 days after the hemispherotomy, with a tendency to minimize the motor performance deficit over time. CONCLUSIONS The present findings show that the technique reproduced neural disconnection with minimal resection of brain parenchyma in young adult rats, thereby duplicating the hemispherotomy procedures in human patients. (AU)

• Processo FAPESP: 07/01174-1 - Estudo do envolvimento do sistema opióide, serotoninérgico e noradrenérgico do sistema endógeno de inibição de dor em processos antinociceptivos induzidos por reações de fuga orientada evocada pela estimulação química do hipotálamo medial
• Beneficiário: Norberto Cysne Coimbra
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/11855-8 - Estudo da influência de endocanabinoides do corpo estriado e do mesencéfalo ventral na atividade das projeções GABAérgicas neoestriado-nigro-tectais durante as reações de pânico de roedores ameaçados por serpentes peçonhentas
• Beneficiário: Norberto Cysne Coimbra
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 02/01497-1 - Estudo do envolvimento do núcleo magno da rafe, do núcleo gigantocelular e núcleo pré-tectal anterior, e de receptores opiódes, kappa e delta serotoninérgicos 5HT2/1C, e noradrenérgicos alfa, beta, na analgesia pós-cital: um estudo neuroanatômico e neuropsicofisiológico em um modelo experimental de epilepsia
• Beneficiário: Daoud Hibrahim Elias Filho
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 12/03798-0 - Envolvimento de receptores opióides e endocanabinóides da substância negra na modulação da atividade da via GABAérgica nigro-tectal durante a organização do comportamento de defesa eliciado por roedores confrontados com serpentes veneníferas
• Beneficiário: Norberto Cysne Coimbra
• Modalidade de apoio: Auxílio à Pesquisa - Regular