Marigold carotenoids: Much more than lutein esters

Autor(es)

Rodrigues, Daniele Bobrowski ^[1] ; Mercadante, Adriana Zerlotti ^[1] ; Barros Mariutti, Lilian Regina ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Food Engn, Dept Food Sci, BR-13083862 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Fac Food Engn, Dept Food & Nutr, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food Research International; v. 119, p. 653-664, MAY 2019.
• Citações Web of Science: 1

Resumo

Carotenoids constitute a large group of lipophilic pigments whose health-promoting benefits have been widely recognized. Hydroxy-containing carotenoids can be found in both free form or esterified with fatty acids in several plant matrices, but the native carotenoid profile is overall poorly explored due to the difficulty of analyzing carotenoid esters. One of the main natural sources of carotenoids is the marigold flower, which has been extensively used by the industry for the production of food colorants or supplements, both often manufactured with no saponification process. Although lutein esters are well established as the major compounds naturally found in marigold petals and their products, carotenoid esters other than the lutein ones have not been extensively examined. We carried out a comprehensive identification of carotenoids and carotenoid esters from marigold petals by LC-DAD-(APCI + )MS/MS. Whereas 18 carotenoids were identified in the saponified extract, 56 were identified when no saponification procedure was carried out: 6 free carotenoids, 20 monoesters and 30 diesters. This is the first time that esters of zeaxanthin, violaxanthin, auroxanthin, zeinoxanthin and beta-cryptoxanthin are identified in marigold. The structural information obtained through characteristic fragmentation patterns and diagnostic fragments in MS and MS/MS spectra (APCI+) sustained the differentiation between carotenoid esters with similar characteristics. Therefore, the separation of carotenoids by reversed-phase liquid chromatography using C-30 columns in combination with DAD and APCI-MS/MS detection allowed high sensitivity and selectivity for carotenoid ester analysis. (AU)

• Processo FAPESP: 13/23218-1 - Bioacessibilidade in vitro de carotenoides e de seus ésteres em murici e influência da presença de compostos fenólicos na bioacessibilidade de carotenoides em sistema mimetizador de alimentos
• Beneficiário: Daniele Bobrowski Rodrigues
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/15238-8 - Influência de compostos fenólicos na bioacessibilidade in vitro, assimilação e transporte celular de carotenóides por células Caco-2
• Beneficiário: Daniele Bobrowski Rodrigues
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado