Molecular analysis of clonally related Salmonella Typhi recovered from epidemiologically unrelated cases of typhoid fever, Brazil

Autor(es)

Tiba-Casas, Monique Ribeiro ^[1] ; Sacchi, Claudio Tavares ^[1] ; Goncalves, Claudia Regina ^[1] ; Almeida, Elisabete Aparecida ^[1] ; Soares, Flavia Barrosa ^[1] ; de Jesus Bertani, Amanda Maria ^[1] ; Fernandes, Sueli Aparecida ^[1] ; de Paula Eduardo, Maria Bernadete ^[2] ; Camargo, Carlos Henrique ^[1]

Afiliação do(s) autor(es)

^[1] Adolfo Lutz Inst, Ave Dr Arnaldo 351, 9 Andar, BR-01246000 Sao Paulo - Brazil

^[2] Ctr Vigilancia Epidemiol Prof Alexandre Vranjac, Coordenadoria Controle Doencas, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES; v. 81, p. 191-195, APR 2019.
• Citações Web of Science: 0

Resumo

Background: The primary method of molecular subtyping for the identification and investigation of outbreaks has been pulsed-field gel electrophoresis (PFGE). In some cases, this technique has not been able to show discrimination between the unrelated strains that can be achieved by whole genome sequencing (WGS). Methods: The aim of this study was to determine the strengths and drawbacks of WGS using different analytic approaches compared to traditional typing method, PFGE, for retrospectively typing clusters cases of 28 S. Typhi. Results: We evaluated three analytical approaches on the WGS data set (Nucleotide Difference (ND), (SNPs) and Whole genome multi locus sequence typing (wgMLST) that identically classified the clusters-related strains into two clusters, cluster A (with strains from 2017), and Cluster B (with strains from 2007). Conclusions: In this study WGS based typing, was able to compete with PFGE for differentiation of the clusters of S. Typhi strains. (C) 2019 The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. (AU)

• Processo FAPESP: 19/02534-9 - Análise Molecular de Salmonella Typhi clonalmente relacionadas provenientes de casos de febre tifóide não relacionados epidemiologicamente no Brasil.
• Beneficiário: Monique Ribeiro Tiba Casas
• Modalidade de apoio: Auxílio à Pesquisa - Publicações científicas - Artigo