Linear Mitochondrial Genome in Anthozoa (Cnidaria): A Case Study in Ceriantharia

Autor(es)

Stampar, Sergio N. ^[1] ; Broe, Michael B. ^[2] ; Macrander, Jason ^{[3, 4]} ; Reitzel, Adam M. ^[3] ; Brugler, Mercer R. ^{[5, 6]} ; Daly, Marymegan ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Fac Ciencias & Letras, Dept Ciencias Biol, Assis, SP - Brazil

^[2] Ohio State Univ, Dept Evolut Ecol & Organismal Biol, Columbus, OH 43210 - USA

^[3] Univ North Carolina Charlotte, Dept Biol Sci, Charlotte, NC - USA

^[4] Florida Southern Coll, Dept Biol, Lakeland, FL - USA

^[5] CUNY, NYC Coll Technol, Biol Sci Dept, 285 Jay St, Brooklyn, NY 11201 - USA

^[6] Amer Museum Nat Hist, Dept Invertebrate Zool, Cent Pk West & 79th St, New York, NY 10024 - USA

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 9, APR 15 2019.
• Citações Web of Science: 2

Resumo

Sequences and structural attributes of mitochondrial genomes have played a critical role in the clarification of relationships among Cnidaria, a key phylum of early-diverging animals. Among the major lineages of Cnidaria, Ceriantharia ({''}tube anemones{''}) remains one of the most enigmatic in terms of its phylogenetic position. We sequenced the mitochondrial genomes of two ceriantharians to see whether the complete organellar genome would provide more support for the phylogenetic placement of Ceriantharia. For both Isarachnanthus nocturnus and Pachycerianthus magnus, the mitochondrial gene sequences could not be assembled into a single circular genome. Instead, our analyses suggest that both species have mitochondrial genomes consisting of multiple linear fragments. Linear mitogenomes are characteristic of members of Medusozoa, one of the major lineages of Cnidaria, but are unreported for Anthozoa, which includes the Ceriantharia. The inferred number of fragments and variation in gene order between species is much greater within Ceriantharia than among the lineages of Medusozoa. We identify origins of replication for each of the five putative chromosomes of the Isarachnanthus nocturnus mitogenome and for each of the eight putative chromosomes of the Pachycerianthus magnus mitogenome. At 80,923 bp, I. nocturnus now holds the record for the largest animal mitochondrial genome reported to date. The novelty of the mitogenomic structure in Ceriantharia highlights the distinctiveness of this lineage but, because it appears to be both unique to and diverse within Ceriantharia, it is uninformative about the phylogenetic position of Ceriantharia relative to other Anthozoa. The presence of tRNA(Met) and tRNA(Trp) in both ceriantharian mitogenomes supports a closer relationship between Ceriantharia and Hexacorallia than between Ceriantharia and any other cnidarian lineage, but phylogenetic analysis of the genes contained in the mitogenomes suggests that Ceriantharia is sister to a clade containing Octocorallia + Hexacorallia indicating a possible suppression of tRNA(Trp) in Octocorallia. (AU)

• Processo FAPESP: 15/24408-4 - Evolução e diversidade de Ceriantharia (Cnidaria)
• Beneficiário: Sérgio Nascimento Stampar
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/50028-0 - Effects of diet and diel lighting on expression of venom diversity in two species of tube dwelling anemone
• Beneficiário: Sérgio Nascimento Stampar
• Modalidade de apoio: Auxílio à Pesquisa - Regular