Lignocellulolytic characterization and comparative secretome analysis of a Trichoderma erinaceum strain isolated from decaying sugarcane straw

Autor(es):Mostrar menos -

da Silva, Desiree S. ^[1] ; Dantzger, Miriam ^[1] ; Assis, Michelle A. ^[2] ; Gallardo, Jessica C. M. ^[1] ; Teixeira, Gleidson S. ^{[1, 2, 3]} ; Missawa, Silvia K. ^[1] ; Domingues, Romenia R. ^[4] ; Carazzolle, Marcelo F. ^[2] ; Lunardi, Ines ^[1] ; Leme, Adriana F. P. ^[4] ; Pereira, Goncalo A. G. ^{[1, 2]} ; Parreiras, Lucas S. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] GranBio BioCelere, BR-13098321 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Genet Evolut Microbiol & Immunol Dept, Genom & Express Lab LGE, BR-13083859 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Food Engn Dept, Bioproc & Metab Engn Lab LEMeB, BR-13083859 Campinas, SP - Brazil

^[4] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biosci Natl Lab LNBio, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FUNGAL BIOLOGY; v. 123, n. 4, p. 330-340, APR 2019.
• Citações Web of Science: 0

Resumo

The fungus Trichoderma reesei is employed in the production of most enzyme cocktails used by the lignocellulosic biofuels industry today. Despite significant improvements, the cost of the required enzyme preparations remains high, representing a major obstacle for the industrial production of these alternative fuels. In this study, a new Trichoderma erinaceum strain was isolated from decaying sugarcane straw. The enzyme cocktail secreted by the new isolate during growth in pretreated sugarcane straw-containing medium presented higher specific activities of beta-glucosidase, endoxylanase, beta-xylosidase and alpha-galactosidase than the cocktail of a wild T. reesei strain and yielded more glucose in the hydrolysis of pretreated sugarcane straw. A proteomic analysis of the two strains' secretomes identified a total of 86 proteins, of which 48 were exclusive to T erinaceum, 35 were exclusive to T. reesei and only 3 were common to both strains. The secretome of T. erinaceum also displayed a higher number of carbohydrate-active enzymes than that of T. reesei (37 and 27 enzymes, respectively). Altogether, these results reveal the significant potential of the T. erinaceum species for the production of lignocellulases, both as a possible source of enzymes for the supplementation of industrial cocktails and as a candidate chassis for enzyme production. (C) 2019 British Mycological Society. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 16/02792-0 - Identificação de enzimas com potencial biotecnológico em um fungo do gênero Trichoderma
• Beneficiário: Michelle Alexandrino de Assis
• Modalidade de apoio: Bolsas no Brasil - Mestrado