Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Lignocellulolytic characterization and comparative secretome analysis of a Trichoderma erinaceum strain isolated from decaying sugarcane straw

Full text
Author(s):
Show less -
da Silva, Desiree S. [1] ; Dantzger, Miriam [1] ; Assis, Michelle A. [2] ; Gallardo, Jessica C. M. [1] ; Teixeira, Gleidson S. [1, 2, 3] ; Missawa, Silvia K. [1] ; Domingues, Romenia R. [4] ; Carazzolle, Marcelo F. [2] ; Lunardi, Ines [1] ; Leme, Adriana F. P. [4] ; Pereira, Goncalo A. G. [1, 2] ; Parreiras, Lucas S. [1, 2]
Total Authors: 12
Affiliation:
[1] GranBio BioCelere, BR-13098321 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Genet Evolut Microbiol & Immunol Dept, Genom & Express Lab LGE, BR-13083859 Campinas, SP - Brazil
[3] Univ Estadual Campinas, Food Engn Dept, Bioproc & Metab Engn Lab LEMeB, BR-13083859 Campinas, SP - Brazil
[4] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biosci Natl Lab LNBio, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: FUNGAL BIOLOGY; v. 123, n. 4, p. 330-340, APR 2019.
Web of Science Citations: 0
Abstract

The fungus Trichoderma reesei is employed in the production of most enzyme cocktails used by the lignocellulosic biofuels industry today. Despite significant improvements, the cost of the required enzyme preparations remains high, representing a major obstacle for the industrial production of these alternative fuels. In this study, a new Trichoderma erinaceum strain was isolated from decaying sugarcane straw. The enzyme cocktail secreted by the new isolate during growth in pretreated sugarcane straw-containing medium presented higher specific activities of beta-glucosidase, endoxylanase, beta-xylosidase and alpha-galactosidase than the cocktail of a wild T. reesei strain and yielded more glucose in the hydrolysis of pretreated sugarcane straw. A proteomic analysis of the two strains' secretomes identified a total of 86 proteins, of which 48 were exclusive to T erinaceum, 35 were exclusive to T. reesei and only 3 were common to both strains. The secretome of T. erinaceum also displayed a higher number of carbohydrate-active enzymes than that of T. reesei (37 and 27 enzymes, respectively). Altogether, these results reveal the significant potential of the T. erinaceum species for the production of lignocellulases, both as a possible source of enzymes for the supplementation of industrial cocktails and as a candidate chassis for enzyme production. (C) 2019 British Mycological Society. Published by Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 16/02792-0 - Identification of enzymes with potential for biotechnological applications in a Trichoderma sp fungus
Grantee:Michelle Alexandrino de Assis
Support Opportunities: Scholarships in Brazil - Master