Host cell protein LAMP-2 is the receptor for Trypanosoma cruzi surface molecule gp82 that mediates invasion

Autor(es)

Ferreira Rodrigues, Joao Paulo ^[1] ; Onofre, Thiago Souza ^[1] ; Barbosa, Bruno Couto ^[1] ; Ferreira, Eden Ramalho ^[1] ; Bonfim-Melo, Alexis ^[1] ; Yoshida, Nobuko ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, R Pedro de Toledo 669, 6 Andar, BR-04039032 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Cellular Microbiology; v. 21, n. 5 MAY 2019.
• Citações Web of Science: 1

Resumo

Host cell invasion by Trypanosoma cruzi metacyclic trypomastigote (MT) is mediated by MT-specific surface molecule gp82, which binds to a still unidentified receptor, inducing lysosome spreading and exocytosis required for the parasitophorous vacuole formation. We examined the involvement of the major lysosome membrane-associated LAMP proteins in MT invasion. First, human epithelial HeLa cells were incubated with MT in the presence of antibody to LAMP-1 or LAMP-2. Antibody to LAMP-2, but not to LAMP-1, significantly reduced MT invasion. Next, HeLa cells depleted in LAMP-1 or LAMP-2 were generated. Cells deficient in LAMP-2, but not in LAMP-1, were significantly more resistant to MT invasion than wild-type controls. The possibility that LAMP-2 might be the receptor for gp82 was examined by co-immunoprecipitation assays. Protein A/G magnetic beads cross-linked with antibody directed to LAMP-1 or LAMP-2 were incubated with HeLa cell and MT detergent extracts. Gp82 bound to LAMP-2 but not to LAMP-1. Binding of the recombinant gp82 protein to wild-type and LAMP-1-deficient cells, which was dose dependent and saturable, had a similar profile and was much higher as compared with LAMP-2-depleted cells. These data indicate that MT invasion is accomplished through recognition of gp82 by its receptor LAMP-2. (AU)

• Processo FAPESP: 16/15000-4 - Trypanosoma cruzi: variabilidade genômica intra- e interespecífica e mecanismos de invasão/evasão celular
• Beneficiário: Renato Arruda Mortara
• Modalidade de apoio: Auxílio à Pesquisa - Temático