Systemic injection of an H4 receptor agonist induces a decrease in CREB and pCREB levels in the cerebellar vermis and prefrontal cortex in mice

Autor(es)

Fernandes, C. E. M. ^[1] ; Serafim, K. R. ^[1] ; Gianlorenco, A. C. L. ^[1] ; Mattioli, R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Lab Neurociencia, Dept Fisioterapia, Ctr Biol Ciencias & Saude, Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Medical and Biological Research; v. 52, n. 5 2019.
• Citações Web of Science: 0

Resumo

Studies have shown that an injection with the histamine H4 receptor agonist VUF-8430 modulates emotional memory processes. In the present study, the aim was to verify if intraperitoneal (ip) injection of VUF-8430 (500 ng/kg) in mice affects the synthesis of proteins required for memory consolidation processes by activating the phosphorylation of CREB (pCREB) in classical structures linked to emotional memory (prefrontal cortex, amygdala, and hippocampus) and the cerebellar vermis, a structure that has also been recently implicated in emotional memory. The results obtained using western blot analysis demonstrated that VUF-8430 induced a decrease in CREB and pCREB levels in the cerebellar vermis and prefrontal cortex, suggesting that this dose impaired the activation of cell signaling pathways in these structures. There was no change in protein expression in the amygdala and hippocampus. Our results are preliminary, and further investigations are needed to investigate the role of the H4 receptors in the central nervous system. (AU)

• Processo FAPESP: 15/22309-9 - A participação do receptor histaminérgico h4 na modulação da memória emocional e no controle motor em camundongos.
• Beneficiário: Carlos Eduardo Monici Fernandes
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/15342-0 - A participação do receptor histaminérgico h4 na modulação da memória emocional e no controle motor em camundongos.
• Beneficiário: Rosana Mattioli
• Modalidade de apoio: Auxílio à Pesquisa - Regular