Autor(es)

Ruy, Patricia De Cassia ^[1] ; Monteiro-Teles, Natalia Melquie ^[1] ; Miserani Magalhaes, Rubens Daniel ^[1] ; Freitas-Castro, Felipe ^[1] ; Dias, Leandro ^[1] ; Aquino Defina, Tania Paula ^[1] ; De Vasconcelos, Elton Jose Rosas ^{[2, 3]} ; Myler, Peter J. ^[2] ; Cruz, Angela Kaysel ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Cell & Mol Biol Dept, Sao Paulo - Brazil

^[2] Ctr Infect DiseaseRes, Washington, DC - USA

^[3] Univ Leeds, Fac Biol Sci, Sch Mol & Cell Biol, Leeds Omics, Leeds, W Yorkshire - England

• Tipo de documento: Artigo Científico
• Fonte: RNA BIOLOGY; v. 16, n. 5, p. 639-660, MAY 4 2019.
• Citações Web of Science: 3

Resumo

Leishmaniasis is a worldwide public health problem caused by protozoan parasites of the genus Leishmania. Leishmania braziliensis is the most important species responsible for tegumentary leishmaniases in Brazil. An understanding of the molecular mechanisms underlying the success of this parasite is urgently needed. An in-depth study on the modulation of gene expression across the life cycle stages of L. braziliensis covering coding and noncoding RNAs (ncRNAs) was missing and is presented herein. Analyses of differentially expressed (DE) genes revealed that most prominent differences were observed between the transcriptomes of insect and mammalian proliferative forms (6,576 genes). Gene ontology (GO) analysis indicated stage-specific enriched biological processes. A computational pipeline and 5 ncRNA predictors allowed the identification of 11,372 putative ncRNAs. Most of the DE ncRNAs were found between the transcriptomes of insect and mammalian proliferative stages (38%). Of the DE ncRNAs, 295 were DE in all three stages and displayed a wide range of lengths, chromosomal distributions and locations; many of them had a distinct expression profile compared to that of their protein-coding neighbors. Thirty-five putative ncRNAs were submitted to northern blotting analysis, and one or more hybridization-positive signals were observed in 22 of these ncRNAs. This work presents an overview of the L. braziliensis transcriptome and its adjustments throughout development. In addition to determining the general features of the transcriptome at each life stage and the profile of protein-coding transcripts, we identified and characterized a variety of noncoding transcripts. The novel putative ncRNAs uncovered in L. braziliensis might be regulatory elements to be further investigated. (AU)

• Processo FAPESP: 13/50219-9 - Investigação sobre fatores e mecanismos do controle de expressão gênica em Leishmania: do papel de modificações pós-traducionais, RNAs não codificadores, cis-elementos e amplificação gênica
• Beneficiário: Angela Kaysel Cruz
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/16429-4 - Bioinformática na análise de expressão gênica em Leishmania
• Beneficiário: Patrícia de Cássia Ruy
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 15/16684-1 - Investigação de RNAs não codificadores em Leishmania (Viannia) braziliensis
• Beneficiário: Natália Melquie Monteiro Teles
• Modalidade de apoio: Bolsas no Brasil - Doutorado