Development of an Enzyme-Linked Immunosorbent Assay To Detect Antibodies Targeting Recombinant Envelope Protein 2 of Mayaro Virus

Autor(es)

Fumagalli, Marcilio Jorge ^[1] ; de Souza, William Marciel ^[1] ; Romeiro, Marilia Farignoli ^[1] ; de Souza Costa, Michell Charles ^[2] ; Slhessarenko, Renata Dezengrini ^[2] ; Moraes Figueiredo, Luiz Tadeu ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Virol Res Ctr, Ribeirao Preto, SP - Brazil

^[2] Univ Fed Mato Grosso, Med Sci Fac, Cuiaba, Mato Grosso - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Clinical Microbiology; v. 57, n. 5 MAY 2019.
• Citações Web of Science: 4

Resumo

Mayaro virus (MAYV) is a neglected arthropod-borne virus (arbovirus) antigenically clustered into the Semliki Forest complex group of Alphavirus genus (Togaviridae family), maintained in an unclear zoonotic cycle involving mosquitoes from Haemagogus genus as the main vector. The genome is composed of a positive single-stranded RNA of 11.5 kb in length, which contains two genes that encode four nonstructural (nsP1 to nsP4) and five structural (C, E3, E2, 6K, and E1) proteins. In the present study, we have developed an enzyme-linked immunosorbent assay (ELISA) using as antigen the recombinant envelope protein 2 of MAYV produced in an Escherichia coli system (rE2-MAYV ELISAs). A panel of 68 human serum samples from suspected arboviral cases was analyzed and titrated for anti-MAYV IgM and IgG antibody detection. The rE2-MAYV ELISA detected 33.8% (23/68) IgG-positive samples, demonstrating 100% sensitivity and 78.95% specificity compared to the MAYV-specific 50% plaque reduction neutralization assay. In addition, the positive MAYV-neutralizing samples showed high titers of detection by rE2-MAYV ELISA, suggesting a highly sensitive test. The rE2-MAYV ELISA also detected 42.5% (29/68) IgM-positive samples, of which 13.8% (4/29) presented high-avidity interactions with rE2-MAYV. Cross-reactivity was observed with Chikungunya virus (CHIKV)-specific murine antibody sample but not with CHIKV-specific human and other Alphavirus murine antibodies. In short, we have developed a rapid, simple, specific, and sensitive MAYV rE2-ELISA, and our preliminary results show its potential applicability to diagnosis of MAYV infections. (AU)

• Processo FAPESP: 17/13981-0 - Caracterização, genômica e diagnóstico de vírus com importância em saúde pública no Brasil por sequenciamento de alto desempenho
• Beneficiário: William Marciel de Souza
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/01414-1 - Produção de peptídeos recombinantes virais antigênicos e desenvolvimento de métodos sorológicos para diagnóstico de infecções pelos vírus Chikungunya, Mayaro e Zika
• Beneficiário: Marcilio Jorge Fumagalli
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/02438-6 - Estudos com Bunyaviridae causadores de doença
• Beneficiário: Luiz Tadeu Moraes Figueiredo
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/24150-9 - Pesquisa de vírus em roedores silvestres, mosquitos e carrapatos
• Beneficiário: William Marciel de Souza
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/20851-8 - Estudo sobre infecções por Alphavirus e Flavivirus em amostras de Banco de Sangue
• Beneficiário: Marilia Farignoli Romeiro
• Linha de fomento: Bolsas no Brasil - Doutorado