The Dynamics of Replication in Trypanosoma cruzi Parasites by Single-Molecule Analysis

Autor(es)

de Araujo, Christiane B. ^{[1, 2]} ; Calderano, Simone G. ^{[3, 2]} ; Elias, Maria Carolina ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Especial Ciclo Celular, Ave Vital Brasil 1500, BR-05503900 Sao Paulo - Brazil

^[2] Inst Butantan, Ctr Toxins Immune Response & Cell Signaling CeTIC, Ave Vital Brasil 1500, BR-05503900 Sao Paulo - Brazil

^[3] Inst Butantan, Lab Parasitol, Ave Vital Brasil 1500, BR-05503900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Eukaryotic Microbiology; v. 66, n. 3, p. 514-518, MAY-JUN 2019.
• Citações Web of Science: 0

Resumo

Here, we investigated the features of replication in Trypanosoma cruzi epimastigotes based on fork speed progression, which is influenced by distinct features such as DNA polymerase rate, susceptibility to DNA damage and repair, secondary structures, transcription and chromatin state. Although T. cruzi exhibits a mean fork speed (2.05 +/- 0.10 kb/min) very similar to other trypanosomatids, we found that the majority of DNA molecules replicated more slowly, with a frequency distribution approximately 1 kb/min. This frequency distribution analysis provides more information about the replication profile of this organism. (AU)

• Processo FAPESP: 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular
• Beneficiário: Hugo Aguirre Armelin
• Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 16/50050-2 - How do common and diverged features of the replicative stress response shape the biology of TriTryp parasites?
• Beneficiário: Maria Carolina Quartim Barbosa Elias Sabbaga
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/13375-5 - Origens da replicação em tripanossomas
• Beneficiário: Christiane Bezerra de Araujo
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado