Divergent coronaviruses detected in wild birds in Brazil, including a central park in SAo Paulo

Autor(es):Mostrar menos -

Barbosa, Carla M. ^[1] ; Durigon, Edison L. ^[1] ; Thomazelli, Luciano M. ^[1] ; Ometto, Tatiana ^[1] ; Marcatti, Roberta ^[2] ; Nardi, Marcello Shiavo ^[2] ; de Aguiar, Daniel M. ^[3] ; Pinho, Joao Batista ^[4] ; Petry, Maria Virginia ^[5] ; Neto, Isaac Simao ^[6] ; Serafini, Patricia ^[7] ; Rodrigues, Roberta Costa ^[8] ; de Azevedo Junior, Severino Mendes ^[9] ; Goes, Luiz Gustavo B. ^[1] ; de Araujo, Jansen ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, 1374 Prof Lineu Prestes Ave, BR-05508900 Sao Paulo - Brazil

^[2] Sao Paulo Municipal, Wildlife & Green Areas Dept, Sao Paulo - Brazil

^[3] Univ Fed Mato Grosso, Hosp Vet, Lab Virol & Rickettsioses, Cuiaba, Mato Grosso - Brazil

^[4] Univ Fed Mato Grosso, Inst Biociencias, Lab Ecol Aves, Cuiaba, Mato Grosso - Brazil

^[5] Univ Vale Rio Sinos UNISINOS, Sao Leopoldo, RS - Brazil

^[6] Inst Chico Mendes Conservacao Biodiversidade ICMB, Brasilia, DF - Brazil

^[7] Ctr Nacl Pesquisa & Conservacao Aves Silvestres C, Cabedelo, Paraiba - Brazil

^[8] Univ Fed Paraiba UFPB, Joao Pessoa, Paraiba - Brazil

^[9] Univ Fed Rural Pernambuco UFRPE, Recife, PE - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 50, n. 2, p. 547-556, APR 2019.
• Citações Web of Science: 0

Resumo

Coronaviruses are single-stranded positive-sense RNA viruses associated with important avian diseases. Their relatively high rates of mutation and recombination frequencies allow them to adapt to new hosts and ecological niches. Although Brazil has 18% of global avian species diversity, studies regarding the presence of avian viral diseases in wild birds in South America are scarce. In this study, we performed a retrospective analysis of the presence of CoVs in 746 wild birds. Oropharyngeal and cloacal swabs were obtained and placed together in vials containing VTM transport medium collected in different regions of Brazil between 2006 and 2013. Screening for viral nucleic acid was performed using conventional RT-PCR and pancoronavirus nested PCR. Positive samples were characterized by partial sequencing of the RNA-dependent RNA polymerase (RdRp) gene, and ensuing phylogenetic analysis was performed to investigate the association between virus epidemiology and bird migration routes. Coronavirus RNA were detected and sequenced from six samples, in which three were related to gammacoronaviruses group and the other three to deltacoronavirus group. Our study documents the presence of CoVs related to avian gamma- and deltacoronaviruses circulating in both urban- and poultry-farm regions of Brazil, implicating wild birds as potential carriers of CoVs which may represent a risk to poultry farms and public health in Brazil. (AU)

• Processo FAPESP: 13/05485-2 - Estudo do vírus da Doença de Newcastle pelo método de RT-PCR em tempo real, em aves silvestres de diferentes regiões do Brasil e da Antártica
• Beneficiário: Luciano Matsumiya Thomazelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/05994-9 - Monitoramento do Vírus do Oeste do Nilo no Brasil
• Beneficiário: Tatiana Ometto de Araujo
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 11/13821-7 - Eco-epidemiologia comparada do vírus da influenza aviária em aves migratórias no território brasileiro
• Beneficiário: Jansen de Araujo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/03172-0 - Eco-epidemiologia do vírus do Oeste do Nilo e outros flavivirus circulantes na fauna pantaneira
• Beneficiário: Tatiana Ometto de Araujo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado