Revisiting Polybia paulista wasp venom using shotgun proteomics - Insights into the N-linked glycosylated venom proteins

Autor(es)

de Souza, Caroline Lacerra ^[1] ; Aparecido dos Santos-Pinto, Jose Roberto ^[1] ; Esteves, Franciele Grego ^[1] ; Perez-Riverol, Amilcar ^[1] ; Romani Fernandes, Luis Gustavo ^[2] ; Zollner, Ricardo de Lima ^[2] ; Palma, Mario Sergio ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Inst Biosci Rio Claro, Dept Biol, Ctr Study Social Insects, BR-13500 Rio Claro, SP - Brazil

^[2] Univ Campinas UNICAMP, Fac Med, Lab Translat Immunol, Cidade Univ Zeferino Vaz, BR-13083887 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PROTEOMICS; v. 200, p. 60-73, MAY 30 2019.
• Citações Web of Science: 2

Resumo

The partial proteome of Polybia paulista wasp venom was previously reported elsewhere using a gel-dependent approach and resulted in the identification of a limited number of venom toxins. Here, we reinvestigated the P. paulista venom using a gel-free shotgun proteomic approach; the highly dynamic range of this approach facilitated the detection and identification of 1673 proteins, of which 23 venom proteins presented N-linked glycosylation as a posttranslational modification. Three different molecular forms of PLAT were identified as allergenic proteins, and two of these forms were modified by N-linked glycosylation. This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins. It was possible to identify venom toxin-like proteins that are commonly reported in other animal venoms, including arthropods such as spiders and scorpions. Thus, the findings reported here may contribute to improving our understanding of the composition of P. paulista venom, its envenoming mechanism and the pathologies experienced by the victim after the wasp stinging accident. Biological significance: The present study significantly expanded the number of proteins identified in P. paulista venom, contributing to improvements in our understanding of the envenoming mechanism produced by sting accidents caused by this wasp. For example, novel wasp venom neurotoxins have been identified, but no studies have assessed the presence of this type of toxin in social wasp venoms. In addition, 23 N-linked glycosylated venom proteins were identified in the P. paulista venom proteome, and some of these proteins might be relevant allergens that are immunoreactive to human IgE. (AU)

• Processo FAPESP: 17/04680-7 - Análise do perfil glicoproteômico do veneno da vespa social Polybia paulista
• Beneficiário: Caroline Lacerra de Souza
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 16/16212-5 - Proteopeptídeos naturais da fauna, flora e microbiota brasileira como potenciais modelos para o desenvolvimento racional de novos fármacos de uso terapêutico: isolamento, elucidação estrutural, síntese química e ensaios de atividade funcional
• Beneficiário: Mario Sergio Palma
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 17/10373-0 - Perfilagem peptidômica e caracterização estrutural-funcional das vesículas lipídicas presentes na teia da aranha Nephila clavipes
• Beneficiário: Franciele Grego Esteves
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/26451-9 - Bioprospecção e Análise Estrutural das Proteínas da Seda de Artrópodes por uma Abordagem Proteômica Utilizando um Sistema nanoLC-ESI-CID/ETD
• Beneficiário: José Roberto Aparecido dos Santos-Pinto
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/22405-3 - Identificação e síntese de peptídeos correspondentes a epítopos lineares de células-b de alérgenos do veneno de hymenoptera sociais: desenvolvimento de insumos para diagnóstico e imunoterapia de alergia
• Beneficiário: Amilcar Perez Riverol
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado