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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Green synthesis of colloidal gold nanoparticles using latex from Hevea brasiliensis and evaluation of their in vitro cytotoxicity and genotoxicity

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Autor(es):
Santos, Natalia M. [1] ; Gomes, Andressa S. [1] ; Cavalcante, Dalita G. S. M. [1] ; Santos, Luis F. [1] ; Teixeira, Silvio R. [1] ; Cabrera, Flavio C. [1] ; Job, Aldo Eloizo [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] UNESP, FCT, Dept Fis, Rua Cyro Bueno, 40 Jd Morumbi, BR-19060560 Presidente Prudente, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: IET Nanobiotechnology; v. 13, n. 3, p. 307-315, MAY 2019.
Citações Web of Science: 1
Resumo

Latex extracted from Hevea brasiliensis tree has been used as a green alternative for preparing gold nanoparticles (Au NPs); however, no study evaluating the cytotoxic and genotoxic potential of Au NPs synthesised using H. brasiliensis has been published. The present study aimed to synthesise and characterise colloidal Au NPs using latex from H. brasiliensis and to evaluate their in vitro cytotoxicity and genotoxicity. Ideal conditions for the green synthesis of Au NPs were studied. In vitro cytotoxicity and genotoxicity of Au NPs in CHO-K1 cells was also evaluated. Our findings indicated that the ideal synthesis conditions of pH, temperature, reduction time, and concentrations of latex and HAuCl4 were 7.0, 85 degrees C, 120 min, 3.3 mg/mL, and 5.0 mmol/L, respectively. LC50(24 h) of Au NPs was 119.164 +/- 5.31 mu g/mL. Lowest concentration of Au NPs tested presented minimal cytotoxicity and genotoxicity. However, high concentrations of Au NPs promoted DNA damage and cell death via apoptosis. On the basis of these findings, the authors optimised the use of an aqueous solution of H. brasiliensis latex as a reducing/stabilising agent for the green synthesis of Au NPs. Low concentrations of these NPs are biocompatible in normal cell types, suggesting that these NPs may be used in biological applications. (AU)

Processo FAPESP: 14/01774-2 - Síntese verde de nanopartículas de ouro utilizando látex Hevea brasiliensis como agente redutor
Beneficiário:Natália Mariana dos Santos
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 13/24884-5 - Avaliação in vitro da biocompatibilidade de lâmina de borracha Natural/Couro para ser aplicada na indústria têxtil
Beneficiário:Dalita Gomes Silva Morais Cavalcante
Linha de fomento: Bolsas no Brasil - Pós-Doutorado