Investigation of five alpha-hydroxy acids for enamel and dentin etching: Demineralization depth, resin adhesion and dentin enzymatic activity

Autor(es)

Trevelin, Livia Tosi ^{[1, 2]} ; Villanueva, Jose ^[1] ; Zamperini, Camila A. ^[1] ; Mathew, Mathew T. ^[1] ; Matos, Adriana Bona ^[2] ; Bedran-Russo, Ana K. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Illinois, Dept Restorat Dent, Coll Dent, UIC, Chicago, IL 60612 - USA

^[2] Univ Sao Paulo, Dept Operat Dent, Coll Dent, UIC, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Dental Materials; v. 35, n. 6, p. 900-908, JUN 2019.
• Citações Web of Science: 0

Resumo

Objectives. Surface conditioning of enamel and dentin is a key step during adhesive restorative procedures and strategies. The aim of this study was to investigate the effectiveness of five alpha-hydroxy-acids (AHAs) as enamel and dentin surface etchants. Methods. Enamel and dentin specimens were prepared from human molars to determine the depth of demineralization by optical profilometry (Delta z), the resin bond strength to enamel and dentin (mu TBS), the micro-permeability of dentin-resin interfaces, and the gelatinolytic activity of dentin matrix induced by AHAs {[}glycolic (GA), lactic (LA), citric (CA), malic (MI) and tartaric (TA)] and controls {[}phosphoric (PA) and maleic (MA)]. All acids were prepared at 35% concentration. Adhesion studies employed Adper Single Bond Plus bonding system. Data were individually processed and analyzed by ANOVA, post-hoc tests and Pearson correlations (alpha = 0.05). Results. AHA exhibited statistically lower depth of demineralization of enamel and dentin (average 4 fold) than controls (p < 0.001). In enamel, MA and PA etching resulted in higher mu TBS than AHA groups (p < 0.001). In dentin, GA, TA, CI and LA etching resulted in statistically similar mu TBS than PA (p < 0.05). The hybrid-layer (HL) thickness and interfacial micro-permeability intensity were statistically lower for AHA groups (p < 0.05). A significant positive correlation was observed between the intensity of micro-permeability and the thickness of HL (p < 0.05). AHA etchants elicited lower dentin enzymatic activity than controls (p < 0.05). Significance. AHAs effectively etched enamel and dentin surfaces. In particular, GA and TA resulted in suitable mu TBS and sealing ability as well as induced less gelatinolytic activity in dentin than PA and MA. (C) 2019 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 15/12651-1 - Influência da largura temporal de pulso do laser de Er:YAG na morfologia dentinária, na formação da camada híbrida e na resistência adesiva
• Beneficiário: Lívia Tosi Trevelin
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/18118-6 - Novas estratégias para prevenir a degradação da interface adesiva
• Beneficiário: Lívia Tosi Trevelin
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado