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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Differential microRNA profile underlies the divergent healing responses in skin and oral mucosal wounds

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Autor(es):
Simoes, Alyne [1, 2] ; Chen, Lin [1] ; Chen, Zujian [3] ; Zhao, Yan [1] ; Gao, Shang [4] ; Marucha, Phillip T. [1, 5] ; Dai, Yang [4] ; DiPietro, Luisa A. [1, 6] ; Zhou, Xiaofeng [1, 3, 6, 7]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Illinois, Coll Dent, Dept Periodont, Ctr Wound Healing & Tissue Regenerat, Chicago, IL 60607 - USA
[2] Univ Sao Paulo, Sch Dent, Oral Biol Lab, Dept Biomat & Oral Biol, Sao Paulo, SP - Brazil
[3] Univ Illinois, Coll Dent, Dept Periodont, Ctr Mol Biol Oral Dis, Chicago, IL 60607 - USA
[4] Univ Illinois, Coll Engn, Dept Bioengn, Chicago, IL - USA
[5] Oregon Hlth & Sci Univ, Coll Dent, Portland, OR 97201 - USA
[6] Univ Illinois, Grad Coll, Chicago, IL 60607 - USA
[7] Univ Illinois, UIC Canc Ctr, Chicago, IL 60607 - USA
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: SCIENTIFIC REPORTS; v. 9, MAY 9 2019.
Citações Web of Science: 2
Resumo

Oral mucosal wounds heal faster than skin wounds, yet the role of microRNAs in this differential healing has never been examined. To delineate the role of microRNAs in this site-specific injury response, we first compared the microRNAome of uninjured skin and oral mucosa in mice. A total of 53 tissue-specific microRNAs for skin and oral mucosa epithelium were identified. The most striking difference was the high abundance of miR-10a/b in skin (accounting for 21.10% of the skin microRNAome) as compared to their low expression in oral mucosa (2.87%). We further examined the dynamic changes of microRNAome throughout the time course of skin and oral mucosal wound healing. More differentially expressed microRNAs were identified in skin wounds than oral wounds (200 and 33, respectively). More specifically, miR-10a/b was significantly down-regulated in skin but not oral wounds. In contrast, up-regulation of miR-21 was observed in both skin and oral wounds. The therapeutic potential of miR-10b and miR-21 in accelerating wound closure was demonstrated in in vitro assays and in a murine skin wound model. Thus, we provided the first site-specific microRNA profile of skin and oral mucosal wound healing, and demonstrate the feasibility of a microRNA-based therapy for promoting wound closure. (AU)

Processo FAPESP: 16/16332-0 - Papel do microRNA na reparação da pele: efeito da terapia com laser de baixa potência e análise comparativa com a reparação da mucosa oral
Beneficiário:Alyne Simões Gonçalves
Modalidade de apoio: Bolsas no Exterior - Pesquisa