Short-term evaluation of photobiomodulation therapy on the proliferation and undifferentiated status of dental pulp stem cells

Autor(es)

Ferreira, L. S. ^[1] ; Diniz, I. M. A. ^[2] ; Maranduba, C. M. S. ^[3] ; Miyagi, S. P. H. ^[4] ; Rodrigues, M. F. S. D. ^[5] ; Moura-Netto, C. ^[1] ; Marques, Marcia Martins ^{[1, 6]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Restorat Dent, Sch Dent, Sao Paulo, SP - Brazil

^[2] Univ Fed Minas Gerais, Sch Dent, Dept Restorat Dent, Belo Horizonte, MG - Brazil

^[3] Univ Fed Juiz de Fora, Inst Biol Sci, Juiz De Fora, MG - Brazil

^[4] FMU, Sch Dent, Sao Paulo, SP - Brazil

^[5] Univ Nove Julho, Biophoton Appl Hlth Sci, Sao Paulo, SP - Brazil

^[6] Univ Sao Paulo, Dept Dentist, Fac Odontol, Ave Prof Lineu Prestes 2227, BR-05508000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 34, n. 4, p. 659-666, JUN 2019.
• Citações Web of Science: 3

Resumo

The aim of this in vitro study was to analyze the effect of photobiomodulation therapy (PBMT) on the proliferation and undifferentiating status of stem cell from human exfoliated deciduous teeth (SHEDs). PBMT was carried out with an aluminum gallium indium phosphide (InGaAlP) diode laser in contact and punctual mode (continuous wave, 660nm, 20mW, 0.028cm(2), and average energy densities of 1 (1s), 3 (4s), 5 (7s), 10 (14s), 15 (21s), or 20 (28s) J/cm(2) per point). The immunoprofile of the SHEDs was analyzed using flow cytometry. Cell proliferation was assessed by the MTT reduction assay. Gene expressions of mesenchymal stem cell markers (OCT4, Nestin, CD90, and CD105) were assessed by RT-qPCR 48h after PBMT. Data were compared by analysis of variance (ANOVA) and Tukey's test (p0.05). Cells cultured under nutritional deficit and treated with PBMT at 5J/cm(2) presented similar cell growth than those of positive control group. Cell growth was significantly higher than those of other groups. Mesenchymal stem cell gene markers were still expressed after PBMT at 5J/cm(2). In a short-term analysis, PBMT increases the number of stem cells with no interference in the undifferentiated state of the irradiated cells, which opens wide possibilities for application in tissue regeneration. (AU)

• Processo FAPESP: 09/13483-4 - Análise do efeito da fototerapia com laser em baixa intensidade em células-tronco de polpa dentária humana de dente decíduo (SHED-LacZ): Estudo in vitro e in vivo da produção de proteínas da matriz dentinária
• Beneficiário: Cacio de Moura Netto
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado