Short-term evaluation of photobiomodulation therapy on the proliferation and undifferentiated status of dental pulp stem cells

Author(s)

Ferreira, L. S. ^[1] ; Diniz, I. M. A. ^[2] ; Maranduba, C. M. S. ^[3] ; Miyagi, S. P. H. ^[4] ; Rodrigues, M. F. S. D. ^[5] ; Moura-Netto, C. ^[1] ; Marques, Marcia Martins ^{[1, 6]}

Affiliation

^[1] Univ Sao Paulo, Dept Restorat Dent, Sch Dent, Sao Paulo, SP - Brazil

^[2] Univ Fed Minas Gerais, Sch Dent, Dept Restorat Dent, Belo Horizonte, MG - Brazil

^[3] Univ Fed Juiz de Fora, Inst Biol Sci, Juiz De Fora, MG - Brazil

^[4] FMU, Sch Dent, Sao Paulo, SP - Brazil

^[5] Univ Nove Julho, Biophoton Appl Hlth Sci, Sao Paulo, SP - Brazil

^[6] Univ Sao Paulo, Dept Dentist, Fac Odontol, Ave Prof Lineu Prestes 2227, BR-05508000 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Lasers in Medical Science; v. 34, n. 4, p. 659-666, JUN 2019.
• Web of Science Citations: 3

Abstract

The aim of this in vitro study was to analyze the effect of photobiomodulation therapy (PBMT) on the proliferation and undifferentiating status of stem cell from human exfoliated deciduous teeth (SHEDs). PBMT was carried out with an aluminum gallium indium phosphide (InGaAlP) diode laser in contact and punctual mode (continuous wave, 660nm, 20mW, 0.028cm(2), and average energy densities of 1 (1s), 3 (4s), 5 (7s), 10 (14s), 15 (21s), or 20 (28s) J/cm(2) per point). The immunoprofile of the SHEDs was analyzed using flow cytometry. Cell proliferation was assessed by the MTT reduction assay. Gene expressions of mesenchymal stem cell markers (OCT4, Nestin, CD90, and CD105) were assessed by RT-qPCR 48h after PBMT. Data were compared by analysis of variance (ANOVA) and Tukey's test (p0.05). Cells cultured under nutritional deficit and treated with PBMT at 5J/cm(2) presented similar cell growth than those of positive control group. Cell growth was significantly higher than those of other groups. Mesenchymal stem cell gene markers were still expressed after PBMT at 5J/cm(2). In a short-term analysis, PBMT increases the number of stem cells with no interference in the undifferentiated state of the irradiated cells, which opens wide possibilities for application in tissue regeneration. (AU)

• FAPESP's process: 09/13483-4 - Analysis of the effect of low level laser phototherapy on stem cells derived from human deciduous dental pulp (SHED-LacZ): In vitro and in vivo study of dentin matrix protein production
• Grantee: Cacio de Moura Netto
• Support Opportunities: Scholarships in Brazil - Post-Doctoral