Characterization of traumatic spinal cord injury model in relation to neuropathic pain in the rat

Autor(es)

Batista, Chary Marquez ^[1] ; Mariano, Eric Domingos ^[1] ; Onuchic, Fernando ^[1] ; Dale, Camila Squarzoni ^[2] ; dos Santos, Gustavo Bispo ^[3] ; Cristante, Alexandre Fogaca ^[3] ; Otoch, Jose Pinhata ^[4] ; Teixeir, Manoel Jacobsen ^[1] ; Morgalla, Matthias ^[5] ; Lepski, Guilherme ^{[6, 5, 7]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Dept Neurol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Med, Dept Orthoped & Traumatol, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sch Med, Dept Surg, Sao Paulo - Brazil

^[5] Eberhard Karls Univ Tubingen, Dept Neurosurg, Tubingen - Germany

^[6] Univ Sao Paulo, Sch Med, Dept Psychiat, Sao Paulo - Brazil

^[7] Univ Sao Paulo, Dept Psychiat, Room 4220, 4th Floor, Av Dr Arnaldo, 455, BR-01246903 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SOMATOSENSORY AND MOTOR RESEARCH; v. 36, n. 1, p. 14-23, JAN 2 2019.
• Citações Web of Science: 2

Resumo

Purpose/aim: Neuropathic pain following spinal cord injury (SCI) has a tremendous impact on patient's quality of life, and frequently is the most limiting aspect of the disease. In view of the severity of this condition and the absence of effective treatments, the establishment of a reliable animal model that reproduces neuropathic pain after injury is crucial for a better understanding of the pathophysiology and for the development of new therapeutic strategies. Thus, the objective of the present study was to standardize the traumatic SCI model in relation to neuropathic pain.Materials and methods: Wistar rats were submitted to SCI of mild intensity (pendulum height 12.5mm) or moderate intensity (pendulum height 25mm) using the New York University Impactor equipment. Behavioural assessment was performed during 8weeks. Thereafter, spinal cords were processed for immunohistochemistry.Results: The animals of the moderate injury group in comparison with mild injury had a greater motor function deficit, worse mechanical allodynia, and latter bladder recovery; moreover, histological analysis revealed more extensive lesions with lower neuronal population.Conclusions: Our study suggests that moderate SCI causes a progressive and long-lasting painful condition (at least 8weeks), in addition to motor impairment, and thus represents a reliable animal model for the study of chronic neuropathic pain after SCI. (AU)

• Processo FAPESP: 13/12056-0 - Tratamento da dor mielopática por meio de transplante de progenitores neurais em modelo de lesão medular em ratos
• Beneficiário: Guilherme Alves Lepski
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/02154-0 - Avaliação da dor neuropática e das funções motora e somato-sensitiva após o transplante de células-tronco em modelo de lesão da medula espinal em ratos
• Beneficiário: Chary Ely Martin Marquez Batista
• Linha de fomento: Bolsas no Brasil - Doutorado