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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Disruption of giant unilamellar vesicles mimicking cell membranes induced by the pesticides glyphosate and picloram

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Autor(es):
Lemma, Tibebe [1] ; Marques Ruiz, Gilia Cristine [1] ; Oliveira, Jr., Osvaldo N. [2] ; Constantino, Carlos J. L. [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista UNESP, FCT, BR-19060900 Presidente Prudente, SP - Brazil
[2] Univ Sao Paulo, Sao Carlos Inst Phys, CP 369, BR-13560970 Sao Carlos, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Biophysical Chemistry; v. 250, JUL 2019.
Citações Web of Science: 0
Resumo

Giant unilamellar vesicles (GUVs) have been one of the most extensively investigated membrane model to study cell membrane-ligand interactions. In this study, we investigated the interaction between glyphosate and picloram with GUVs made with sphingomyelin (SM), cholesterol (CHOL), and dioleoyl-sn-glycerol-3-phosphocholine (DOPC) (DOPC/SM (1:1), DOPC/CHOL/SM (1:1:1)) in a physiological environment using confocal and phase contrast microscopy. At high pesticide concentrations (70 to 90 mu M), we generally found the GUVs undergoing a physical such as contouring, elongation, and eventually lose their characteristic spherical shape. In addition, to determine the comparative effect of the pesticides, control experiments were performed using GUVs made with only DOPC and DOPC/SM 1:1. The results show that, at low concentration (0.5 mu M), a significant effect was observed during a 30 min incubation time. These findings also suggest that cholesterol may play a significant role in the permeability of the vesicle against the action of the pesticides, which have important biological implications on the lipid composition of the membrane. (AU)

Processo FAPESP: 16/06424-5 - Eletroformação de vesículas unilamelares gigantes aplicadas como sistemas miméticos de biomembranas
Beneficiário:Tibebe Lemma
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 13/14262-7 - Filmes nanoestruturados de materiais de interesse biológico
Beneficiário:Osvaldo Novais de Oliveira Junior
Linha de fomento: Auxílio à Pesquisa - Temático