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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Disruption of giant unilamellar vesicles mimicking cell membranes induced by the pesticides glyphosate and picloram

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Author(s):
Lemma, Tibebe [1] ; Marques Ruiz, Gilia Cristine [1] ; Oliveira, Jr., Osvaldo N. [2] ; Constantino, Carlos J. L. [1]
Total Authors: 4
Affiliation:
[1] Univ Estadual Paulista UNESP, FCT, BR-19060900 Presidente Prudente, SP - Brazil
[2] Univ Sao Paulo, Sao Carlos Inst Phys, CP 369, BR-13560970 Sao Carlos, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Biophysical Chemistry; v. 250, JUL 2019.
Web of Science Citations: 0
Abstract

Giant unilamellar vesicles (GUVs) have been one of the most extensively investigated membrane model to study cell membrane-ligand interactions. In this study, we investigated the interaction between glyphosate and picloram with GUVs made with sphingomyelin (SM), cholesterol (CHOL), and dioleoyl-sn-glycerol-3-phosphocholine (DOPC) (DOPC/SM (1:1), DOPC/CHOL/SM (1:1:1)) in a physiological environment using confocal and phase contrast microscopy. At high pesticide concentrations (70 to 90 mu M), we generally found the GUVs undergoing a physical such as contouring, elongation, and eventually lose their characteristic spherical shape. In addition, to determine the comparative effect of the pesticides, control experiments were performed using GUVs made with only DOPC and DOPC/SM 1:1. The results show that, at low concentration (0.5 mu M), a significant effect was observed during a 30 min incubation time. These findings also suggest that cholesterol may play a significant role in the permeability of the vesicle against the action of the pesticides, which have important biological implications on the lipid composition of the membrane. (AU)

FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
Grantee:Osvaldo Novais de Oliveira Junior
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 16/06424-5 - Electroforming giant unilamellar vesicles applied as biomembranes mimetic systems
Grantee:Tibebe Lemma
Support Opportunities: Scholarships in Brazil - Post-Doctoral