Effect of contamination with Lactobacillus fermentum I2 on ethanol production by Spathaspora passalidarum

Autor(es)

Collograi, Karen Cristina ^{[1, 2]} ; da Costa, Aline Carvalho ^[1] ; Ienczak, Jaciane Lutz ^[3]

Afiliação do(s) autor(es)

^[1] State Univ Campinas UNICAMP, Sch Chem Engn, 500 Albert Einstein Ave, BR-13083852 Campinas, SP - Brazil

^[2] Brazilian Bioethanol Sci & Technol Lab CTBE CNPEM, 10000 Giuseppe Maximo Scolfaro St, BR-13083852 Campinas, SP - Brazil

^[3] Santa Catarina Fed Univ, Chem Engn & Food Engn Dept, CP 476, BR-88040900 Florianopolis, SC - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Applied Microbiology and Biotechnology; v. 103, n. 12, p. 5039-5050, JUN 2019.
• Citações Web of Science: 0

Resumo

Second-generation bioethanol is a promising source of renewable energy. In Brazilian mills, the production of ethanol from sugarcane (first generation, 1G) is a consolidated process performed by Saccharomyces cerevisiae and characterized by high substrate concentrations, high cell density, and cell recycle. The main bacterial contaminants in 1G fermentation tanks are lactic acid bacteria, especially bacteria from the Lactobacillus genus, which is associated with a decrease in ethanol yield and yeast cell viability, among other negative effects. Second-generation (2G) bioethanol production is characterized by the conversion of glucose and xylose into ethanol by genetically modified or non-Saccharomyces yeasts. Spathaspora passalidarum is a promising non-Saccharomyces yeast for 2G ethanol production due to its ability to effectively convert xylose into ethanol. The effect of bacterial contamination on the fermentation of this yeast is unknown; therefore, L. fermentum, a common bacterium found in Brazilian 1G processes, was studied in coculture with S. passalidarum in a fed-batch fermentation process similar to that used in 1G mills. Individually, L. fermentum I2 was able to simultaneously consume glucose and xylose in nutrient-rich broth (Man, Rogosa, and Sharpe (MRS + xylose) but failed to grow in a glucose- and xylose-based synthetic broth. In coculture with S. passalidarum, the bacteria remained at a concentration of 10(8)UFC/mL throughout cell recycling, but no flocculation was observed, and it did not affect the fermentative parameters or the cellular viability of the yeast. Under both conditions, the maximum ethanol production was 21gL(-1) with volumetric productivity ranging from 0.65 to 0.70gL(-1)h(-1). S. passalidarum was thus shown to be resistant to L. fermentum I2 under the conditions studied. (AU)

• Processo FAPESP: 16/06142-0 - Estratégias para melhorar o desempenho do processo de produção de etanol de segunda geração
• Beneficiário: Aline Carvalho da Costa
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular
• Processo FAPESP: 17/04997-0 - Avaliação de leveduras nativas Scheffersomyces stipitis e Spathaspora passalidarum na produção de bioetanol de segunda geração a partir de hidrolisados de bagaço de cana-de-açúcar contendo xilose e glicose
• Beneficiário: Jaciane Lutz Ienczak
• Modalidade de apoio: Auxílio à Pesquisa - Regular