Development and Evaluation of a 66-Well Plate Using a Porous Sorbent in a Four-Phase Extraction Assisted by Electric Field Approach

Autor(es)

Orlando, Ricardo Mathias ^[1] ; Nascentes, Clesia Cristina ; Botelho, Bruno Goncalves ; Moreira, Juliane Soares ; Costa, Karina Araujo ; de Miranda Boratto, Victor Hugo

Afiliação do(s) autor(es)

^[1] Univ Fed Minas Gerais, Dept Chem, Lab Microfluid & Separat, LaMS, BR-30123970 Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Analytical Chemistry; v. 91, n. 10, p. 6471-6478, MAY 21 2019.
• Citações Web of Science: 2

Resumo

In this work, cellulose cone tips were used as a hydrophilic sorbent supporting an aqueous electrolyte solution in a four-phase electric field-assisted extraction. The wetted tips were immersed in an immiscible organic phase, acting as a filter to extract analytes present in an aqueous sample. Simultaneous four-phase extractions were carried out under the influence of an electric field using a 66-well plate constructed with inexpensive and readily available laboratory materials. Several parameters that control the electric resistivity of the system were evaluated, and the application of electric potential difference. In a final application, a cationic dye and its metabolite, crystal and leuco crystal violet, were extracted from cone tips provided reproducible and stable electric current during spiked fish extracts and quantified by a digital image analysis method. The method presented a linear range of 2-100 ng mL(-1) (r > 0.999), limit of detection of 1.37 ng mL(-1), inter- (3 consecutive days) and intraday precision determined for 4 concentration levels (n = 6, each) were lower than 29% and mean recoveries for these concentrations ranging from 95% to 115%. The results demonstrated that the 66-well plate provides a stable, reproducible, and simple way to conduct extractions of ionic compounds from complex aqueous matrixes. The effectiveness of the low-cost cellulose cone tips used as a solid fourth phase also confirmed that it can easily overcome many drawbacks of similar liquid-liquid-liquid approaches. (AU)

• Processo FAPESP: 14/50951-4 - INCT 2014: Tecnologias Analíticas Avançadas
• Beneficiário: Celio Pasquini
• Modalidade de apoio: Auxílio à Pesquisa - Temático