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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Effect of analogues of cationic peptides on dentin mineralization markers in odontoblast-like cells

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Autor(es):
Caiaffa, Karina S. [1, 2] ; Basso, Fernanda. G. [3] ; Santos-Filho, Norival A. [4, 5] ; de Souza-Costa, Carlos Alberto [3] ; Sakai, Vivien T. [6] ; Cilli, Eduardo M. [5] ; Duque, Cristiane [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista UNESP, Aracatuba Dent Sch, Dept Pediat Dent & Publ Hlth, R Jose Bonifacio, 1193, BR-16015050 Aracatuba, SP - Brazil
[2] State Univ Sao Paulo UNESP, Aracatuba Sch Dent, Dept Endodont, Aracatuba, SP - Brazil
[3] State Univ Sao Paulo UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, Aracatuba, SP - Brazil
[4] State Univ Sao Paulo UNESP, Inst Chem, Dept Biochem & Chem Technol, Aracatuba, SP - Brazil
[5] Sao Paulo State Univ UNESP, Registro Expt Campus, Registro, SP - Brazil
[6] Fed Univ Alfenas UNIFAL, Sch Dent, Dept Clin & Surg, Alfenas, SP - Brazil
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: ARCHIVES OF ORAL BIOLOGY; v. 103, p. 19-25, JUL 2019.
Citações Web of Science: 0
Resumo

Objectives: To evaluate the effect of analogues of cationic peptides on the viability and the expression of phenotypic and genotypic markers of dentin mineralization in MDPC-23 odontoblast-like cells. Materials and methods: Cells were exposed to serial dilutions of analogues of cationic peptides hBD-3-1e and KR-12-a5 compared to peptide LL-37 and their viability was assessed by methyltetrazolium assay. Next, peptides (0.78-62.5 mu g/mL) were applied on the MDPC-23 cells for evaluating the total protein (TP) production, alkaline phosphatase (ALP) activity and mineralized nodule deposition. Gene expression of mineralization markers (DSPP and DMP-1) was also determined by quantitative PCR. Results: LL-37 and hBD-3-1C(V) treatment did not affect cellular viability at concentrations below 62.5 mu g/mL. KR-12-a5 reduced cell viability above 31.25 mu g/mL. TP production was similar for all groups compared with the control group, except by hBD-3-1C(V) (at 15.62 mu g/mL). LL-37 (at 62.5 mu g/mL) induced higher ALP activity than control and other experimental groups. LL-37 and hBD-3-1C(V), at 62.5 mu g/mL and KR-12-a5 at 31.25 mu g/mL stimulated the highest deposition of mineralized nodule. Overall, no statistical differences were observed between the groups for DSPP-1 and DMP-1 expressions. Conclusions: LL-37 was the only peptide that induced both ALP activity and mineralized nodules deposition, without affecting cell viability. None of peptides tested induced the expression of DSPP or DMP-1, genes commonly involved in active dentin mineralization. (AU)

Processo FAPESP: 13/24606-5 - Efeito antimicrobiano de análogos de peptídeos catiônicos sobre microrganismos de importância endodôntica e sua influência na expressão gênica de marcadores de mineralização dentinária
Beneficiário:Karina Sampaio Caiaffa
Linha de fomento: Bolsas no Brasil - Mestrado