Effect of analogues of cationic peptides on dentin mineralization markers in odontoblast-like cells

Author(s)

Caiaffa, Karina S. ^{[1, 2]} ; Basso, Fernanda. G. ^[3] ; Santos-Filho, Norival A. ^{[4, 5]} ; de Souza-Costa, Carlos Alberto ^[3] ; Sakai, Vivien T. ^[6] ; Cilli, Eduardo M. ^[5] ; Duque, Cristiane ^[1]

Affiliation

^[1] Univ Estadual Paulista UNESP, Aracatuba Dent Sch, Dept Pediat Dent & Publ Hlth, R Jose Bonifacio, 1193, BR-16015050 Aracatuba, SP - Brazil

^[2] State Univ Sao Paulo UNESP, Aracatuba Sch Dent, Dept Endodont, Aracatuba, SP - Brazil

^[3] State Univ Sao Paulo UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, Aracatuba, SP - Brazil

^[4] State Univ Sao Paulo UNESP, Inst Chem, Dept Biochem & Chem Technol, Aracatuba, SP - Brazil

^[5] Sao Paulo State Univ UNESP, Registro Expt Campus, Registro, SP - Brazil

^[6] Fed Univ Alfenas UNIFAL, Sch Dent, Dept Clin & Surg, Alfenas, SP - Brazil

• Document type: Journal article
• Source: ARCHIVES OF ORAL BIOLOGY; v. 103, p. 19-25, JUL 2019.
• Web of Science Citations: 0

Abstract

Objectives: To evaluate the effect of analogues of cationic peptides on the viability and the expression of phenotypic and genotypic markers of dentin mineralization in MDPC-23 odontoblast-like cells. Materials and methods: Cells were exposed to serial dilutions of analogues of cationic peptides hBD-3-1e and KR-12-a5 compared to peptide LL-37 and their viability was assessed by methyltetrazolium assay. Next, peptides (0.78-62.5 mu g/mL) were applied on the MDPC-23 cells for evaluating the total protein (TP) production, alkaline phosphatase (ALP) activity and mineralized nodule deposition. Gene expression of mineralization markers (DSPP and DMP-1) was also determined by quantitative PCR. Results: LL-37 and hBD-3-1C(V) treatment did not affect cellular viability at concentrations below 62.5 mu g/mL. KR-12-a5 reduced cell viability above 31.25 mu g/mL. TP production was similar for all groups compared with the control group, except by hBD-3-1C(V) (at 15.62 mu g/mL). LL-37 (at 62.5 mu g/mL) induced higher ALP activity than control and other experimental groups. LL-37 and hBD-3-1C(V), at 62.5 mu g/mL and KR-12-a5 at 31.25 mu g/mL stimulated the highest deposition of mineralized nodule. Overall, no statistical differences were observed between the groups for DSPP-1 and DMP-1 expressions. Conclusions: LL-37 was the only peptide that induced both ALP activity and mineralized nodules deposition, without affecting cell viability. None of peptides tested induced the expression of DSPP or DMP-1, genes commonly involved in active dentin mineralization. (AU)

• FAPESP's process: 13/24606-5 - Antimicrobial effect of cationic peptides analogs on endodontic microrganisms and its influence on gene expression of dentin mineralization markers
• Grantee: Karina Sampaio Caiaffa
• Support Opportunities: Scholarships in Brazil - Master