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Combined effect of KR-12-a5 and EGCG on viability and mineralization potential in pulp cells for endodontic application

Grant number: 19/27317-0
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): July 01, 2020
Effective date (End): April 30, 2021
Field of knowledge:Health Sciences - Dentistry - Pediatric Dentistry
Principal Investigator:Karina Sampaio Caiaffa
Grantee:Larissa de Souza Oliveira
Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil


Bioactive compounds such as antimicrobial cationic peptides and plant derivatives such as flavonoids have been highlighted in the literature due to their therapeutic range. The aim of this study is to evaluate the in vitro evaluation of cell viability and induction capacity of dentin mineralization of the combination of antimicrobial peptide KR-12-a5 to EGCG (Epigallocathecin gallate). First, we intend to evaluate the cell viability in combination of KR-12-a5 and EGCG on pulp undifferentiated cells (hDPC) by assays using the Alamar Blue® method. In addition, the mineralization inducing capacity of the combination will be evaluated by alkaline phosphatase (ALP) assays, total protein production (PT) and mineralized nodule formation (NM - Alizarin red dye assay). With this study, we intend to propose an alternative of combined natural compounds that have interesting therapeutic properties as active ingredient of endodontic medication.