Antimicrobial effect of cationic peptides analogs on endodontic microrganisms and its influence on gene expression of dentin mineralization markers

• Grant number: 13/24606-5
• Support type: Scholarships in Brazil - Master
• Effective date (Start): June 01, 2014
• Effective date (End): June 30, 2015
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal researcher: Cristiane Duque
• Grantee: Karina Sampaio Caiaffa
• Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Endodontic treatment of young permanent teeth due to trauma or local infection before the normal physiological closure of the root apex represents a real clinical challenge. Cationic antimicrobial peptides and their synthetic analogs have been studied as alternatives to traditional antibiotics in eliminating microorganisms. The objective of this study is to evaluate the antimicrobial activity of planktonic and biofilm culture and determine the effect of cationic peptides analogs on the expression of genes related to the process of dentine mineralization. In order to evaluate the inhibitory activity of human defensin -3 (hBD -3- 1CV) and human LL-37 peptide (KR12 - a5) analogs, and the controls chlorhexidine and LL -37, the following standard strains will be used in this study: Enterococcus faecalis, Streptococcus mutans, Actinomyces israelii and Candida albicans. The minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of each peptide analog will be determined. Biofilms of E. faecalis and C. albicans will be exposed to MBC obtained from each group, peptide analogs or controls. The effect of these peptide analogs on dentin mineralization will be analyzed by means of odontoblast cell cultures - MDPC -23 - exposed to MBC of each peptide, and followed by the analysis of the expression of DSPP and DMP - 1 genes by qPCR, alkaline phosphatase activity and production of mineralized nodules. The data analysis will be aided by a statistical computer program, considering p<0.05. This work aims to contribute to Pediatric Dentistry and Endodontic with the study of new biological alternatives for the elimination of infections and promotion of tissue regeneration of immature teeth. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAIAFFA, KARINA S.; BASSO, FERNANDA. G.; SANTOS-FILHO, NORIVAL A.; DE SOUZA-COSTA, CARLOS ALBERTO; SAKAI, VIVIEN T.; CILLI, EDUARDO M.; DUQUE, CRISTIANE. Effect of analogues of cationic peptides on dentin mineralization markers in odontoblast-like cells. ARCHIVES OF ORAL BIOLOGY, v. 103, p. 19-25, JUL 2019. Web of Science Citations: 0.

CAIAFFA, KARINA SAMPAIO; MASSUNARI, LOIANE; DANELON, MARCELLE; ABUNA, GABRIEL FLORES; LOMBARDO BEDRAN, TELMA BLANCA; SANTOS-FILHO, NORIVAL ALVES; PALOMARI SPOLIDORIO, DENISE MADALENA; VIZOTO, NATALIA LEAL; CILLI, EDUARDO MAFFUD; DUQUE, CRISTIANE. KR-12-a5 is a non-cytotoxic agent with potent antimicrobial effects against oral pathogens. BIOFOULING, v. 33, n. 10, p. 807-818, 2017. Web of Science Citations: 5.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CAIAFFA, Karina Sampaio. Efeito citotóxico e antimicrobiano de análogos de peptídeos catiônicos e sua influencia na expressão de marcadores fenotípicos e genotípicos de mineralização dentinária. 2015. Master's Dissertation - Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Odontologia (Campus de Araçatuba)..