Neuroinflammation in the NTS is associated with changes in cardiovascular reflexes during systemic inflammation

Autor(es)

Amorim, Mateus R. ^[1] ; de Deus, Junia L. ^[2] ; Cazuza, Rafael A. ^[3] ; Mota, Clarissa M. D. ^[2] ; da Silva, Luiz E. V. ^[2] ; Borges, Gabriela S. ^[2] ; Batalhao, Marcelo E. ^[4] ; Carnio, Evelin C. ^[4] ; Branco, Luiz G. S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dent Sch Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, BR-14049900 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Sch Philosophy Sci & Literature Ribeirao Pret, BR-14040901 Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Nursing Sch Ribeirao Preto, BR-14040902 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF NEUROINFLAMMATION; v. 16, JUN 20 2019.
• Citações Web of Science: 1

Resumo

BackgroundLipopolysaccharide (LPS)-induced systemic inflammation (SI) is associated with neuroinflammation in the brain, hypotension, tachycardia, and multiple organs dysfunctions. Considering that during SI these important cardiovascular and inflammatory changes take place, we measured the sensitivity of the cardiovascular reflexes baroreflex, chemoreflex, and Bezold-Jarisch that are key regulators of hemodynamic function. We also evaluated neuroinflammation in the nucleus tractus solitarius (NTS), the first synaptic station that integrates peripheral signals arising from the cardiovascular and inflammatory status.MethodsWe combined cardiovascular recordings, immunofluorescence, and assays of inflammatory markers in male Wistar rats that receive iv administration of LPS (1.5 or 2.5mgkg(-1)) to investigate putative interactions of the neuroinflammation in the NTS and in the anteroventral preoptic region of the hypothalamus (AVPO) with the short-term regulation of blood pressure and heart rate.ResultsLPS induced hypotension, tachycardia, autonomic disbalance, hypothermia followed by fever, and reduction in spontaneous baroreflex gain. On the other hand, during SI, the bradycardic component of Bezold-Jarisch and chemoreflex activation was increased. These changes were associated with a higher number of activated microglia and interleukin (IL)-1 levels in the NTS.ConclusionsThe present data are consistent with the notion that during SI and neuroinflammation in the NTS, rats have a reduced baroreflex gain, combined with an enhancement of the bradycardic component of Bezold-Jarisch and chemoreflex despite the important cardiovascular impairments (hypotension and tachycardia). These changes in the cardiac component of Bezold-Jarisch and chemoreflex may be beneficial during SI and indicate that the improvement of theses reflexes responsiveness though specific nerve stimulations may be useful in the management of sepsis. (AU)

• Processo FAPESP: 17/09878-0 - Efeito da estimulação vagal durante desafio imune por LPS em ratos normotensos e espontaneamente hipertensos
• Beneficiário: Mateus Ramos Amorim
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/01633-8 - Papel da serotonina na área pré-óptica na modulação da temperatura corporal e da nocicepção durante a inflamação sistêmica
• Beneficiário: Clarissa Maria Dias Mota
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/17681-9 - Alterações fisiopatológicas durante a inflamação sistêmica
• Beneficiário: Luiz Guilherme de Siqueira Branco
• Modalidade de apoio: Auxílio à Pesquisa - Temático