Molecular, serological, pathological, immunohistochemical and microbiological investigation of Brucella spp. in marine mammals of Brazil reveals new cetacean hosts

Autor(es):Mostrar menos -

Sanchez-Sarmiento, Angelica M. ^[1] ; Carvalho, Vitor L. ^[2] ; Diaz-Delgado, Josue ^[1] ; Ressio, Rodrigo A. ^[3] ; Fernandes, Natalia C. C. A. ^[3] ; Guerra, Juliana M. ^[3] ; Sacristan, Carlos ^[1] ; Groch, Katia R. ^[1] ; Silvestre-Perez, Natalia ^[1] ; Ferreira-Machado, Eduardo ^[1] ; Costa-Silva, Samira ^[1] ; Navas-Suarez, Pedro ^[1] ; Meirelles, Ana C. O. ^[2] ; Favero, Cintia ^[1] ; Marigo, Juliana ^[1] ; Bertozzi, Carolina P. ^[4] ; Colosio, Adriana C. ^[5] ; Marcondes, Milton C. C. ^[5] ; Cremer, Marta J. ^[6] ; dos Santos Silva, Nairleia ^[7] ; Ferreira Neto, Jose Soares ^[7] ; Keid, Lara B. ^[8] ; Soares, Rodrigo ^[7] ; Sierra, Eva ^[9] ; Fernandez, Antonio ^[9] ; Catao-Dias, Jose L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Lab Wildlife Comparat Pathol, Dept Pathol, Sch Vet Med & Anim Sci, Sao Paulo - Brazil

^[2] Assoc Pesquisa & Preservacao Ecossistemas Aquat A, Caucaia - Brazil

^[3] Adolfo Lutz Inst, Sao Paulo - Brazil

^[4] Univ Estadual Paulista UNESP, CLP, IB, Sao Vicente - Brazil

^[5] Inst Baleia Jubarte, Caravelas - Brazil

^[6] Univ Regiao Joinville, Lab Ecol & Conservacao Tetrapodes Marinhos & Cost, Sao Francisco Do Sul - Brazil

^[7] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Prevent Vet Med & Anim Hlth, Sao Paulo - Brazil

^[8] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Pirassununga - Brazil

^[9] Univ Las Palmas Gran Canaria, Sch Vet Med, Inst Anim Hlth & Food Hyg, Arucas, Gran Canaria - Spain

• Tipo de documento: Artigo Científico
• Fonte: TRANSBOUNDARY AND EMERGING DISEASES; v. 66, n. 4, p. 1674-1692, JUL 2019.
• Citações Web of Science: 1

Resumo

Brucella-exposure and infection is increasingly recognized in marine mammals worldwide. To better understand the epidemiology and health impacts of Brucella spp. in marine mammals of Brazil, molecular (conventional PCR and/or real-time PCR), serological (Rose Bengal Test {[}RBT], Competitive {[}c]ELISA, Serum Agglutination Test {[}SAT]), pathological, immunohistochemical (IHC) and/or microbiological investigations were conducted in samples of 129 stranded or by-caught marine mammals (orders Cetartiodactyla {[}n = 124], Carnivora {[}n = 4] and Sirenia {[}n = 1]). Previous serological tests performed on available sera of 27 of the 129 animals (26 cetaceans and one manatee), indicated 10 seropositive cetaceans. Conventional PCR and/or real-time PCR performed in cases with available organs (n = 119) and/or blood or swabs (n = 10) revealed 4/129 (3.1%) Brucella-infected cetaceans (one of them with positive serology; the remaining three with no available sera). Pathological, IHC and/or microbiological analyses conducted in PCR/real-time PCR and/or seropositive cases (n = 13) revealed Brucella-type lesions, including meningitis/meningoencephalitis, pneumonia, necrotizing hepatitis, pericarditis and osteoarthritis in some of those animals, and positive IHC was found in all of them (excepting two live-stranded animals without available organs). Brucella spp. culture attempts were unsuccessful. Our results demonstrated exposure, asymptomatic, acute and chronic Brucella sp. infection in several cetacean species in the Brazilian coast, highlighting the role of this pathogen in stranding and/or death, particularly in Clymene dolphin (Stenella clymene) and short-finned pilot whale (Globicephala macrorhynchus) off Ceara State. Novel hosts susceptible to Brucella included the franciscana (Pontoporia blainvillei), the Guiana dolphin (Sotalia guianensis) and the spinner dolphin (Stenella longirostris). Additionally, three coinfection cases involving Brucella spp. and cetacean morbillivirus, Edwarsiella tarda and Proteus mirabilis were detected. To the best of our knowledge, this is the first long-term and large-scale survey of Brucella spp. in marine mammals of South America, widening the spectrum of susceptible hosts and geographical distribution range of this agent with zoonotic potential. (AU)

• Processo FAPESP: 14/24932-2 - Pesquisa e caracterização da Morbilivirose em cetáceos do Brasil
• Beneficiário: Kátia Regina Groch
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/00735-6 - Pesquisa e caracterização da Morbilivirose em cetáceos do Brasil
• Beneficiário: Jose Luiz Catao Dias
• Modalidade de apoio: Auxílio à Pesquisa - Regular