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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

The Impact of CO2 Laser Treatment and Acidulated Phosphate Fluoride on Enamel Demineralization and Biofilm Formation

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Autor(es):
de Araujo Loiola, Ana Barbara [1] ; Aires, Carolina Patricia [2] ; Curylofo-Zotti, Fabiana Almeida [1] ; Rodrigues Junior, Antonio Luiz [3] ; Souza-Gabriel, Aline Evangelista [1] ; Milori Corona, Silmara Aparecida [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dent Sch Ribeirao Preto, Dept Restorat Dent, Av Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Phys & Chem, BR-14040904 Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Med Sch Ribeirao Preto, Dept Social Med, BR-14049900 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF LASERS IN MEDICAL SCIENCES; v. 10, n. 3, p. 200-206, SUM 2019.
Citações Web of Science: 0
Resumo

Introduction: This study evaluated the impact of CO2 laser treatment and acidulated phosphate fluoride (APF) on enamel demineralization and biofilm formation, using in vitro and in situ designs. Methods: Demineralized enamel slabs were distributed among 8 groups: placebo, placebo + continuous CO2 laser, placebo + repeated CO2 laser, placebo + ultrapulsed CO2 laser, 1.23% APF, APF + continuous CO2 laser, APF + repeated CO2 laser and APF + ultrapulsed CO2 laser. In the in vitro study, 15 enamel slabs from each group were subjected to a pH-cycling regimen for 14 days. In the cross over in situ design, 11 volunteers wore palatal appliances with demineralized enamel slabs for 2 periods of 14 days each. Drops of sucrose solution were dripped onto enamel slabs 8x/day. Biofilms formed on slabs were collected and the colony-forming units (CFU) of Streptococcus mutans and Lactobacillus were determined. Results: For both in vitro and in situ studies, there was no significant difference between treatments (P > 0.05). However, all treatments increased microhardness of demineralized enamel (P < 0.05). After a further in situ cariogenic challenge, with the exception of the placebo, all treatments maintained microhardness values (P < 0.05). Microbiological analysis showed no difference in Streptococcus mutans (P > 0.05) or Lactobacillus (P > 0.05) counts between groups. Conclusion: The results suggest that APF gel combined with the CO2 laser, regardless of the pulse emission mode used, was effective in controlling enamel demineralization, but none of the tested treatments was able to prevent bacterial colonization. (AU)

Processo FAPESP: 12/01414-0 - Influência do modo de emissão do pulso do laser de CO2 e do flúor fosfato acidulado no controle da desmineralização do esmalte: análise in vitro e in situ
Beneficiário:Silmara Aparecida Milori Corona
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 11/05363-9 - Influência do modo de emissão do pulso do laser de CO2 e do flúor fosfato acidulado no controle da desmineralização do esmalte: análise in vitro e in situ
Beneficiário:Ana Bárbara de Araújo Loiola
Linha de fomento: Bolsas no Brasil - Mestrado