Sperm-borne miR-216b modulates cell proliferation during early embryo development via K-RAS

Autor(es)

Rodrigues Alves, Maira Bianchi ^[1] ; de Arruda, Rubens Paes ^[1] ; De Bem, Tiago Henrique Camara ^[2] ; Florez-Rodriguez, Shirley Andrea ^[1] ; de Sa Filho, Manoel Francisco ^{[1, 3]} ; Belleannee, Clemence ^[4] ; Meirelles, Flavio Vieira ^[2] ; da Silveira, Juliano Coelho ^[2] ; Perecin, Felipe ^[2] ; Carvalho Celeghini, Eneiva Carla ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Pirassununga, SP - Brazil

^[2] Univ Sao Paulo, Sch Anim Sci & Food Engn, Dept Vet Med, Pirassununga, SP - Brazil

^[3] Alta Genet Brazil, Uberaba, MG - Brazil

^[4] Univ Laval, Dept Obstet Gynecol & Reprod, Quebec City, PQ - Canada

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 9, JUL 17 2019.
• Citações Web of Science: 0

Resumo

Semen fertilizing potential is dependent upon the morphological, functional and molecular attributes of sperm. Sperm microRNAs (miRNAs) were recently shown to hold promise regarding their association with different fertility phenotypes. However, their role in fertility regulation remains to be determined. We postulated that sperm miRNAs might regulate early embryonic development. From this perspective, sperm quality and 380 sperm miRNAs were investigated in frozen-thawed semen from high (HF; 54.3 +/- 1.0% pregnancy rate) and low (LF; 41.5 +/- 2.3%) fertility bulls. Out of nine miRNAs that showed different levels in sperm cells, miR-216b was present at lower levels in HF sperm cells and zygotes. Among miR-216b target genes (K-RAS, BECN1 and JUN), K-RAS, related to cell proliferation, revealed a higher level in HF two-cell embryos. First cleavage rate, blastocyst cell number and division number were also higher in HF. In addition, by using a model based on polyspermy embryos, we demonstrated an increase in miR-216b levels in zygotes associated with sperm cell entry. Our results shed light on a possible mechanism of paternal contribution involving sperm-borne miR-216b that modulates levels of miR-216b in zygotes and K-RAS in two-cell embryos. This modulation might regulate early development by interfering with the first cleavage and blastocyst quality. (AU)

• Processo FAPESP: 16/05395-1 - Perfil de microRNAs presentes no sêmen e embriões bovinos e sua relação com a fertilidade
• Beneficiário: Eneiva Carla Carvalho Celeghini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/09154-6 - Perfil de microRNAs presentes no sêmen e embriões bovinos e sua relação com a fertilidade
• Beneficiário: Maíra Bianchi Rodrigues Alves
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/23243-4 - Perfil de microRNAs de espermatozóides humanos e murinos coletados de diferentes partes do sistema reprodutivo
• Beneficiário: Maíra Bianchi Rodrigues Alves
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado