Comparative genome analysis of Phyllosticta citricarpa and Phyllosticta capitalensis, two fungi species that share the same host

Autor(es)

Rodrigues, Carolina Munari ^[1] ; Takita, Marco Aurelio ^[1] ; Silva, Nicholas Vinicius ^[2] ; Ribeiro-Alves, Marcelo ^[3] ; Machado, Marcos Antonio ^[1]

Afiliação do(s) autor(es)

^[1] Ctr APTA Citros Sylvio Moreira, Lab Biotecnol, CP4, BR-13490970 Cordeiropolis, SP - Brazil

^[2] Univ Estadual Campinas, Lab Genom & BioEnergia, BR-13083970 Campinas, SP - Brazil

^[3] Fundacao Oswaldo Cruz, Inst Nacl Infectol Evandro Chagas, Lab Pesquisa Clin DST AIDS, BR-21040900 Rio De Janeiro, RJ - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC Genomics; v. 20, JUL 5 2019.
• Citações Web of Science: 2

Resumo

BackgroundCitrus are among the most important crops in the world. However, there are many diseases that affect Citrus caused by different pathogens. Citrus also hosts many symbiotic microorganisms in a relationship that may be advantageous for both organisms. The fungi Phyllosticta citricarpa, responsible for citrus black spot, and Phyllosticta capitalensis, an endophytic species, are examples of closely related species with different behavior in citrus. Both species are always biologically associated and are morphologically very similar, and comparing their genomes could help understanding the different lifestyles. In this study, a comparison was carried to identify genetic differences that could help us to understand the biology of P. citricarpa and P. capitalensis.ResultsDrafts genomes were assembled with sizes close to 33Mb for both fungi, carrying 15,206 and 14,797 coding sequences for P. citricarpa and P. capitalensis, respectively. Even though the functional categories of these coding sequences is similar, enrichment analysis showed that the pathogenic species presents growth and development genes that may be necessary for the pathogenicity of P. citricarpa. On the other hand, family expansion analyses showed the plasticity of the genome of these species. Particular families are expanded in the genome of an ancestor of P. capitalensis and a recent expansion can also be detected among this species. Additionally, evolution could be driven by environmental cues in P. citricarpa.ConclusionsThis work demonstrated genomic differences between P. citricarpa and P. capitalensis. Although the idea that these differences could explain the different lifestyles of these fungi, we were not able to confirm this hypothesis. Genome evolution seems to be of real importance among the Phyllosticta isolates and it is leading to different biological characteristics of these species. (AU)

• Processo FAPESP: 08/57909-2 - Plataforma genômica aplicada ao melhoramento de citros
• Beneficiário: Marcos Antonio Machado
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/50880-0 - INCT 2014: de genômica comparativa e funcional e melhoramento assistido de citros
• Beneficiário: Marcos Antonio Machado
• Modalidade de apoio: Auxílio à Pesquisa - Temático