A further unique chondroitin sulfate from the shrimp Litopenaeus vannamei with antithrombin activity that modulates acute inflammation

Autor(es)

Palhares, Lais C. G. F. ^{[1, 2]} ; Brito, Adriana S. ^{[3, 2]} ; de Lima, Marcelo A. ^{[4, 5]} ; Nader, Helena B. ^[4] ; London, James A. ^[1] ; Barsukov, Igor L. ^[1] ; Andrade, Giulianna P. V. ^[2] ; Yates, Edwin A. ^[1] ; Chavante, Suely F. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Liverpool, Inst Integrat Biol, Dept Biochem, Liverpool L69 7ZB, Merseyside - England

^[2] Univ Fed Rio Grande do Norte, Dept Bioquim, Programa Posgrad Bioquim & Biol Mol, Natal, RN - Brazil

^[3] Univ Fed Rio Grande do Norte, Fac Ciencias Saude Trairi, Santa Cruz, RN - Brazil

^[4] Univ Fed Sao Paulo, Dept Bioquim, Sao Paulo, SP - Brazil

^[5] Keele Univ, Sch Life Sci, Mol & Struct Biosci, Huxley Bldg, Keele ST5 5BG, Staffs - England

• Tipo de documento: Artigo Científico
• Fonte: Carbohydrate Polymers; v. 222, OCT 15 2019.
• Citações Web of Science: 0

Resumo

The detailed structure of a further Chondroitin Sulfate from Litopenaeus vannamei shrimp (sCS) is described. The backbone structure was established by H-1/C-13 NMR, which identified 3-O-sulfated GlcA, 4-O-sulfated GalNAc, 6-O-sulfated GalNAc, and 4,6-di-O-sulfated GalNAc residues. GlcA is linked to GalNAc 4,6 di S and GlcA 3S is linked to GalNAc 4S, GalNAc 4,6 di-S and GalNAc6S residues. The anticoagulant properties of this sCS were evaluated by activated partial thromboplastin time, anti-IIa, anti-Xa and anti-heparin cofactor II-mediated activities, and sCS failed to stabilise antithrombin in a fluoresence shift assay. The anti-inflammatory effect of sCS was explored using a model of acute peritonitis, followed by leukocyte count and measurement of the cytokines, IL-1 beta, IL-6 and TNF-alpha. The compound showed low clotting effects, but high anti-IIa activity and HCII-mediated thrombin inhibition. Its anti-inflammatory effect was shown by leukocyte recruitment inhibition and a decrease in pro-inflammatory cytokine levels. Although the biological role of sCS remains unknown, its properties indicate that it is suitable for studies of multi-potent molecules obtained from natural sources. (AU)

• Processo FAPESP: 17/14179-3 - Tráfego vesicular na biossíntese do heparam sulfato
• Beneficiário: Ivarne Luis dos Santos Tersariol
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/03964-6 - Glicosaminoglicanos e proteoglicanos: relação estrutura e função
• Beneficiário: Helena Bonciani Nader
• Modalidade de apoio: Auxílio à Pesquisa - Temático