Electrochemical paper-based microfluidic device for high throughput multiplexed analysis

Autor(es)

Fava, Elson Luiz ^[1] ; Silva, Tiago Almeida ^{[1, 2]} ; do Prado, Thiago Martimiano ^[1] ; de Moraes, Fernando Cruz ^[1] ; Faria, Ronaldo Censi ^[1] ; Fatibello-Filho, Orlando ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Chem, POB 676, BR-13560970 Sao Carlos, SP - Brazil

^[2] Fed Ctr Technol Educ Minas Gerais, Dept Met & Chem, BR-35180008 Timoteo, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Talanta; v. 203, p. 280-286, OCT 1 2019.
• Citações Web of Science: 3

Resumo

A disposable microfluidic electrochemical paper-based device for multiplexed analysis based on sixteen independent microfluidic channels with electrochemical detection is proposed. A major advantage of this work was the non-necessary use of a wax printer for devices manufacturing which has a high cost of operation. In addition, a commercial multiplexing module was used that has the multiplexing capability of 8-16 channels and, for the first time using this module, the strategy of multiplexing both the working and reference electrodes were used. These sixteen channels with the respective sensors can be operated employing one or multiple electrochemical techniques with good repeatability and reproducibility for high throughput analysis. As a proof of concept, the electrochemical performance of device was tested with ferrocenecarboxylic acid solution employing cyclic voltammetry, square-wave voltammetry, differential-pulse voltammetry and chronoamperometry. This innovative sensing platform presented capacity of production in large scale and application for clinical tests with safety and short time of assays. A biosensor was constructed using glucose oxidase on the platform for the glucose determination in urine as a non-invasive strategy. The analytical curve was linear in the glucose concentration range from 1.0 x 10(-4) mol L-1 to 4 x 10(-2) mol L-1, with a limit of detection of 3 x 10(-5) mol L-1. (AU)

• Processo FAPESP: 16/10012-4 - Desenvolvimento de dispositivo microfluídico descartável contendo arranjos de biossensores para a determinação multiplexada de espécies de interesse biológico em diferentes matrizes
• Beneficiário: Elson Luiz Fava
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/24053-7 - Desenvolvimentos de métodos eletroquímicos simples para a detecção de biomarcadores visando o diagnóstico de doenças
• Beneficiário: Ronaldo Censi Faria
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/10118-0 - Estudo e aplicação da tecnologia eletroquímica para a análise e a degradação de interferentes endócrinos: materiais, sensores, processos e divulgação científica
• Beneficiário: Marcos Roberto de Vasconcelos Lanza
• Modalidade de apoio: Auxílio à Pesquisa - Temático