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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Neurons-derived extracellular vesicles promote neural differentiation of ADSCs: a model to prevent peripheral nerve degeneration

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Autor(es):
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Santos Roballo, Kelly Cristine [1] ; da Silveira, Juliano Coelho [1] ; Bressan, Fabiana Fernandes [1] ; de Souza, Aline Fernanda [1] ; Pereira, Vitoria Mattos [1] ; Pinzon Porras, Jorge Eliecer [1, 2] ; Ros, Felipe Augusto [1] ; Pulz, Lidia Hildebrand [3] ; Strefezzi, Ricardo de Francisco [3, 1] ; Martins, Daniele dos Santos [1] ; Meirelles, Flavio Vieira [1] ; Ambrosio, Carlos Eduardo [1]
Número total de Autores: 12
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Anim Sci & Food Engn, Vet Med Dept, Av Duque de Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil
[2] Univ Natl Columbia, Fac Vet Med & Anim Sci, Dept Posgrad, Bogota - Colombia
[3] Univ Sao Paulo, Fac Vet Med & Anim Sci, Expt & Comparat Pathol Dept, Av Prof Orlando Marques de Paiva 87, BR-05508010 Sao Paulo, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: SCIENTIFIC REPORTS; v. 9, AUG 1 2019.
Citações Web of Science: 0
Resumo

Potential mechanisms involved in neural differentiation of adipocyte derived stem cells (ADSCs) are still unclear. In the present study, extracellular vesicles (EVs) were tested as a potential mechanism involved in the neuronal differentiation of stem cells. In order to address this, ADSCs and neurons (BRC) were established in primary culture and co-culture at three timepoints. Furthermore, we evaluated protein and transcript levels of differentiated ADSCs from the same timepoints, to confirm phenotype change to neuronal linage. Importantly, neuron-derived EVs cargo and EVs originated from co-culture were analyzed and tested in terms of function, such as gene expression and microRNA levels related to the adult neurogenesis process. Ideal neuron-like cells were identified and, therefore, we speculated the in vivo function of these cells in acute sciatic nerve injury. Overall, our data demonstrated that ADSCs in indirect contact with neurons differentiated into neuron-like cells. Neuron-derived EVs appear to play an important role in this process carrying SNAP25, miR-132 and miR-9. Additionally, in vivo neuron-like cells helped in microenvironment modulation probably preventing peripheral nerve injury degeneration. Consequently, our findings provide new insight of future methods of ADSC induction into neuronal linage to be applied in peripheral nerve (PN) injury. (AU)

Processo FAPESP: 15/17897-9 - Comunicação exossomal na transdiferenciação de células-tronco em co-cultivo com células neuronais
Beneficiário:Kelly Cristine Santos Roballo
Linha de fomento: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 14/22887-0 - Exossomos e microvesículas contendo miRNAs modulam mudanças epigenéticas durante o cultivo in vitro de gametas e embriões em bovinos
Beneficiário:Juliano Coelho da Silveira
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 15/21829-9 - EMU concedido no projeto 2014/22887-0: NanoSight LM10
Beneficiário:Juliano Coelho da Silveira
Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
Processo FAPESP: 13/08135-2 - CTC - Centro de Terapia Celular
Beneficiário:Dimas Tadeu Covas
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs