Neurons-derived extracellular vesicles promote neural differentiation of ADSCs: a model to prevent peripheral nerve degeneration

Autor(es):Mostrar menos -

Santos Roballo, Kelly Cristine ^[1] ; da Silveira, Juliano Coelho ^[1] ; Bressan, Fabiana Fernandes ^[1] ; de Souza, Aline Fernanda ^[1] ; Pereira, Vitoria Mattos ^[1] ; Pinzon Porras, Jorge Eliecer ^{[1, 2]} ; Ros, Felipe Augusto ^[1] ; Pulz, Lidia Hildebrand ^[3] ; Strefezzi, Ricardo de Francisco ^{[3, 1]} ; Martins, Daniele dos Santos ^[1] ; Meirelles, Flavio Vieira ^[1] ; Ambrosio, Carlos Eduardo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Anim Sci & Food Engn, Vet Med Dept, Av Duque de Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil

^[2] Univ Natl Columbia, Fac Vet Med & Anim Sci, Dept Posgrad, Bogota - Colombia

^[3] Univ Sao Paulo, Fac Vet Med & Anim Sci, Expt & Comparat Pathol Dept, Av Prof Orlando Marques de Paiva 87, BR-05508010 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 9, AUG 1 2019.
• Citações Web of Science: 0

Resumo

Potential mechanisms involved in neural differentiation of adipocyte derived stem cells (ADSCs) are still unclear. In the present study, extracellular vesicles (EVs) were tested as a potential mechanism involved in the neuronal differentiation of stem cells. In order to address this, ADSCs and neurons (BRC) were established in primary culture and co-culture at three timepoints. Furthermore, we evaluated protein and transcript levels of differentiated ADSCs from the same timepoints, to confirm phenotype change to neuronal linage. Importantly, neuron-derived EVs cargo and EVs originated from co-culture were analyzed and tested in terms of function, such as gene expression and microRNA levels related to the adult neurogenesis process. Ideal neuron-like cells were identified and, therefore, we speculated the in vivo function of these cells in acute sciatic nerve injury. Overall, our data demonstrated that ADSCs in indirect contact with neurons differentiated into neuron-like cells. Neuron-derived EVs appear to play an important role in this process carrying SNAP25, miR-132 and miR-9. Additionally, in vivo neuron-like cells helped in microenvironment modulation probably preventing peripheral nerve injury degeneration. Consequently, our findings provide new insight of future methods of ADSC induction into neuronal linage to be applied in peripheral nerve (PN) injury. (AU)

• Processo FAPESP: 15/17897-9 - Comunicação exossomal na transdiferenciação de células-tronco em co-cultivo com células neuronais
• Beneficiário: Kelly Cristine Santos Roballo
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 14/22887-0 - Exossomos e microvesículas contendo miRNAs modulam mudanças epigenéticas durante o cultivo in vitro de gametas e embriões em bovinos
• Beneficiário: Juliano Coelho da Silveira
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 15/21829-9 - EMU concedido no projeto 2014/22887-0: NanoSight LM10
• Beneficiário: Juliano Coelho da Silveira
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 13/08135-2 - CTC - Centro de Terapia Celular
• Beneficiário: Dimas Tadeu Covas
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs