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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Neurons-derived extracellular vesicles promote neural differentiation of ADSCs: a model to prevent peripheral nerve degeneration

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Santos Roballo, Kelly Cristine [1] ; da Silveira, Juliano Coelho [1] ; Bressan, Fabiana Fernandes [1] ; de Souza, Aline Fernanda [1] ; Pereira, Vitoria Mattos [1] ; Pinzon Porras, Jorge Eliecer [1, 2] ; Ros, Felipe Augusto [1] ; Pulz, Lidia Hildebrand [3] ; Strefezzi, Ricardo de Francisco [3, 1] ; Martins, Daniele dos Santos [1] ; Meirelles, Flavio Vieira [1] ; Ambrosio, Carlos Eduardo [1]
Total Authors: 12
[1] Univ Sao Paulo, Fac Anim Sci & Food Engn, Vet Med Dept, Av Duque de Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil
[2] Univ Natl Columbia, Fac Vet Med & Anim Sci, Dept Posgrad, Bogota - Colombia
[3] Univ Sao Paulo, Fac Vet Med & Anim Sci, Expt & Comparat Pathol Dept, Av Prof Orlando Marques de Paiva 87, BR-05508010 Sao Paulo, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 9, AUG 1 2019.
Web of Science Citations: 0

Potential mechanisms involved in neural differentiation of adipocyte derived stem cells (ADSCs) are still unclear. In the present study, extracellular vesicles (EVs) were tested as a potential mechanism involved in the neuronal differentiation of stem cells. In order to address this, ADSCs and neurons (BRC) were established in primary culture and co-culture at three timepoints. Furthermore, we evaluated protein and transcript levels of differentiated ADSCs from the same timepoints, to confirm phenotype change to neuronal linage. Importantly, neuron-derived EVs cargo and EVs originated from co-culture were analyzed and tested in terms of function, such as gene expression and microRNA levels related to the adult neurogenesis process. Ideal neuron-like cells were identified and, therefore, we speculated the in vivo function of these cells in acute sciatic nerve injury. Overall, our data demonstrated that ADSCs in indirect contact with neurons differentiated into neuron-like cells. Neuron-derived EVs appear to play an important role in this process carrying SNAP25, miR-132 and miR-9. Additionally, in vivo neuron-like cells helped in microenvironment modulation probably preventing peripheral nerve injury degeneration. Consequently, our findings provide new insight of future methods of ADSC induction into neuronal linage to be applied in peripheral nerve (PN) injury. (AU)

FAPESP's process: 15/17897-9 - Exosomal communication in the stem cells transdifferentiation in co-culture with neuronal cells
Grantee:Kelly Cristine Santos Roballo
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 14/22887-0 - Cell-secreted vesicles containing miRNAs modulate epigenetic changes during in vitro culture of bovine gametes and embryos
Grantee:Juliano Coelho da Silveira
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 15/21829-9 - Multi-user equipment approved in grant 2014/22887-0: Nanosight LM10
Grantee:Juliano Coelho da Silveira
Support type: Multi-user Equipment Program
FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
Grantee:Dimas Tadeu Covas
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC