Multi-user equipment approved in grant 2014/22887-0: Nanosight LM10

• Grant number: 15/21829-9
• Support Opportunities: Multi-user Equipment Program
• Duration: February 01, 2016 - January 31, 2018
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: Juliano Coelho da Silveira
• Grantee: Juliano Coelho da Silveira
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Associated research grant: 14/22887-0 - Cell-secreted vesicles containing miRNAs modulate epigenetic changes during in vitro culture of bovine gametes and embryos, AP.JP
• EMU web page: http://www.fzea.usp.br/?page_id=7918
• Type of equipment: Caracterização de Materiais - Propriedades Físicas - Tamanho de partículas
• Manufacturer: Malvern
• Model: Nanosight NS300

Abstract

The use of assisted reproduction techniques presents an alternative to solve reproductive problems in domestic species and humans. However, there is concern that epigenetic changes occurring during in vitro culture adversely affect tissue programing during fetal development and could lead to a higher pre-disposition to develop heart diseases, reproductive problems, and development of tumors, in adulthood. Epigenetic changes are regulated by DNA methylation, posttranscriptional histone modifications or microRNAs. MicroRNAs are non-coding RNA molecules that regulates gene expression during different tissue developing processes. Recently, cell-secreted vesicles called exosomes and microvesicles carrying bioactive materials including miRNAs were identified in different body fluids. Cell-secreted vesicles are considered a new class of intercellular communication with possible implications in different physiological processes. However, its role during oocyte maturation and embryo development is not well understood. Our main hypothesis that will be tested is that cell-secreted vesicles originating from the reproductive tract modulate epigenetic changes during in vitro culture of gametes and embryos. In order to test this hypothesis cell-secreted vesicles will be isolated from follicular fluid and oviductual cells culture media and added to the oocyte maturation and embryo culture media. The first aim of this project is to determine the relationship between miRNAs and epigenetic changes during oocyte maturation and embryo development in vitro. The second aim of this project is to determine the effects exerted by vesicles secreted by reproductive tract cells on oocyte maturation rates, fertilization and embryo cleavage. The third aim of this proposal is to determine the role of cell-secreted vesicles and miRNAs in oocyte maturation and production of bovine embryos in high and low oxygen tension. Results obtained in this research will establish the role of cell-secreted vesicles by the reproductive tract in regulating epigenetic changes acquired during in vitro culture of oocytes and embryos, generating new therapeutic tools for domestic agriculture species and humans. (AU)

Scientific publications

(13)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAVALCANTE MENDES DE AVILA, ANA CLARA FAQUINELI; BRIDI, ALESSANDRA; ANDRADE, GABRIELLA MAMEDE; DEL COLLADO, MAITE; SANGALLI, JULIANO RODRIGUES; NOCITI, RICARDO PERECIN; DA SILVA JUNIOR, WILSON ARAUJO; BASTIEN, ALEXANDRE; ROBERT, CLAUDE; MEIRELLES, FLAVIO VIEIRA; et al. Estrous cycle impacts microRNA content in extracellular vesicles that modulate bovine cumulus cell transcripts during in vitro maturation. BIOLOGY OF REPRODUCTION, v. 102, n. 2, p. 362-375, FEB 2020. (14/22887-0, 18/14869-2, 17/02037-0, 15/21829-9)

DE AVILA, A. C. F. C. M.; DA SILVEIRA, J. C.. Role of extracellular vesicles during oocyte maturation and early embryo development. REPRODUCTION FERTILITY AND DEVELOPMENT, v. 32, n. 1-2, p. 56-64, 2020. (17/02037-0, 15/21829-9, 14/22887-0)

SANTOS, G.; BOTTINO, M. P.; SANTOS, A. P. C.; SIMOES, L. M. S.; SOUZA, J. C.; FERREIRA, M. B. D.; DA SILVEIRA, J. C.; AVILA, A. C. F. C. M.; BRIDE, A.; SALES, J. N. S.. Subclinical mastitis interferes with ovulation, oocyte and granulosa cell quality in dairy cows. Theriogenology, v. 119, p. 214-219, OCT 1 2018. (14/22887-0, 15/21829-9)

P.C.S. NUNES; S.M. SILVA; R. MAZZARELLA; C.G. LIMA; J.C. SILVEIRA; D.C.A. LEITE-DELLOVA. [Vesículas extracelulares pequenas no soro de cães com sobrepeso e obesos, antes e depois da perda de peso: observações preliminares]. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 75, n. 1, p. 147-152, 2023-02-10. (18/26547-0, 14/22887-0, 19/04981-2, 15/21829-9)

DA SILVEIRA, JULIANO COELHO; ANDRADE, GABRIELLA MAMEDE; SIMAS, ROSINEIDE COSTA; MARTINS-JUNIOR, HELIO ALVES; EBERLIN, MARCOS NOGUEIRA; SMITH, LAWRENCE CHARLES; PERECIN, FELIPE; MEIRELLES, FLAVIO VIEIRA. Lipid profile of extracellular vesicles and their relationship with bovine oocyte developmental competence: New players in intra follicular cell communication. Theriogenology, v. 174, p. 1-8, OCT 15 2021. (11/15416-2, 19/15491-6, 15/21829-9, 12/50533-2, 13/08135-2, 14/22887-0, 14/21034-3)

PEDROSA, ANA CAROLINA; TORRES, MARIANA ANDRADE; ALKMIN, DIEGO VILELA; PINZON, JORGE E. P.; MASSAMI KITAMURA MARTINS, SIMONE MARIA; DA SILVEIRA, JULIANO COELHO; CESAR DE ANDRADE, ANDRE FURUGEN. Spermatozoa and seminal plasma small extracellular vesicles miRNAs as biomarkers of boar semen cryotolerance. Theriogenology, v. 174, p. 60-72, OCT 15 2021. (16/24690-4, 18/12500-1, 15/21829-9, 14/22887-0)

NATÁLIA MARINS BASTOS; JULIANA GERMANO FERST; RODRIGO SILVA GOULART; JULIANO COELHO DA SILVEIRA. The role of the oviduct and extracellular vesicles during early embryo development in bovine. Animal Reproduction, v. 19, n. 1, 2022-04-20. (20/08478-0, 15/21829-9, 20/13075-2, 14/22887-0)

BRIDI, ALESSANDRA; ANDRADE, GABRIELLA M.; DEL COLLADO, MAITE; SANGALLI, JULIANO R.; DE AVILA, ANA C. F. C. M.; MOTTA, IGOR G.; DA SILVA, JULIO C. B.; PUGLIESI, GUILHERME; SILVA, LUCIANO A.; MEIRELLES, FLAVIO V.; et al. mall extracellular vesicles derived from in vivo- or in vitro-produced bovine blastocysts have different miRNAs profiles-Implications for embryo-maternal recognitio. Molecular Reproduction and Development, v. 88, n. 9, AUG 2021. (18/13155-6, 15/21829-9, 17/19681-9, 14/22887-0)

MAZZARELLA, ROSANE; BASTOS, NATALIA MARINS; BRIDI, ALESSANDRA; DEL COLLADO, MAITE; ANDRADE, GABRIELLA MAMEDE; PINZON, JORGE; PRADO, CIBELE MARIA; SILVA, LUCIANO ANDRADE; MEIRELLES, FLAVIO VIEIRA; PUGLIESI, GUILHERME; et al. Changes in Oviductal Cells and Small Extracellular Vesicles miRNAs in Pregnant Cows. FRONTIERS IN VETERINARY SCIENCE, v. 8, MAR 4 2021. (15/21829-9, 19/04981-2, 14/22887-0)

HAMDI, MERIEM; CANON-BELTRAN, KARINA; MAZZARELLA, ROSANE; CAJAS, YULIA N.; LEAL, CLAUDIA L. V.; GUTIERREZ-ADAN, ALFONSO; GONZALEZ, ENCINA M.; DA SILVEIRA, JULIANO C.; RIZOS, DIMITRIOS. Characterization and profiling analysis of bovine oviduct and uterine extracellular vesicles and their miRNA cargo through the estrous cycle. FASEB JOURNAL, v. 35, n. 12, DEC 2021. (17/20339-3, 19/04981-2, 15/21829-9, 14/22887-0)

GARNICA, TAISMARA K.; LESBON, JESSIKA C. C.; AVILA, ANA C. F. C. M.; ROCHETTI, ARINA L.; MATIZ, OSCAR R. S.; RIBEIRO, ROANA C. S.; ZOPPA, ALINE; NISHIYA, ADRIANA T.; COSTA, MIRELA T.; DE NARDI, ANDRIGO B.; et al. Liquid biopsy based on small extracellular vesicles predicts chemotherapy response of canine multicentric lymphomas. SCIENTIFIC REPORTS, v. 10, n. 1, NOV 23 2020. (14/02493-7, 15/21829-9, 17/15406-3, 14/22887-0)

SANTOS ROBALLO, KELLY CRISTINE; DA SILVEIRA, JULIANO COELHO; BRESSAN, FABIANA FERNANDES; DE SOUZA, ALINE FERNANDA; PEREIRA, VITORIA MATTOS; PINZON PORRAS, JORGE ELIECER; ROS, FELIPE AUGUSTO; PULZ, LIDIA HILDEBRAND; STREFEZZI, RICARDO DE FRANCISCO; MARTINS, DANIELE DOS SANTOS; et al. Neurons-derived extracellular vesicles promote neural differentiation of ADSCs: a model to prevent peripheral nerve degeneration. SCIENTIFIC REPORTS, v. 9, AUG 1 2019. (15/17897-9, 14/22887-0, 15/21829-9, 13/08135-2)

ANDRADE, GABRIELLA MAMEDE; BOMFIM, MONALISA MEDRADO; DEL COLLADO, MAITE; MEIRELLES, FLAVIO VIEIRA; PERECIN, FELIPE; DA SILVEIRA, JULIANO COELHO. Oxygen tension modulates extracellular vesicles and its miRNA contents in bovine embryo culture medium. Molecular Reproduction and Development, v. 86, n. 8, p. 1067-1080, AUG 2019. (12/50533-2, 14/21034-3, 15/21829-9, 14/22887-0, 14/21042-6, 13/08135-2)