Exosomal communication in the stem cells transdifferentiation in co-culture with neuronal cells

• Grant number: 15/17897-9
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: March 01, 2016
• End date: September 30, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Carlos Eduardo Ambrósio
• Grantee: Kelly Cristine Santos Roballo
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Associated research grant: 14/22887-0 - Cell-secreted vesicles containing miRNAs modulate epigenetic changes during in vitro culture of bovine gametes and embryos, AP.JP

Abstract

Adipocite Derived Stem Cells (ADSC) in co-culture with neuronal cells can transdifferentiate due to factors originated from neuronal cells, a paracrine signaling state of neuronal cells, probably by exosome. In our study we will observe the transdifferentiation of ADSC, and characterize the neuronal cell types that the ADSC can transdifferentiate; and analyze one of the cellular communication routes in order to explain the cell inducing from one specific cell to the stem cells. Our objective is to understand if transdifferentiation process is possible by only the physical interaction between stem cell and a neuronal cell, no induction medium, as well as studying the exossomal communication as one of the probable cell communication mechanism involved, and, the transdifferentiated stem cells have feature when in vivo. For a better understanding of this cellular reprogramming process, we will use in vivo and in vitro methodologies. In the in vitro study we will use the mouse as an animal model and we will perform innovative techniques for co-culture (stem cells with neural cells) and we will carry out the isolation of exosomes for the analysis of the mechanisms of cell communication and analysis in vitro of the transdifferentiation and functionality os the ADSC by gene expression. In the in vivo study we will perform autologous transplant ADSC in peripheral nerve injury in order to assess whether such cells assist in nerve regeneration. With this project we can better understand the cellular communication process between the stem cells and somatic cells, in addition to expanding the scope of cell therapies for tissue regeneration in the nervous system injuries. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTOS ROBALLO, KELLY CRISTINE; SOUZA, ALINE FERNANDA; LARA, VALERIA MARIA; PINHEIRO, ALESSANDRA OLIVEIRA; GOMES, INGRID DA SILVA; KARAM, RAFAEL GARCIA; MARTINS, DANIELE DOS SANTOS; MACHADO, LUCIANA CRISTINA; AMBROSIO, CARLOS EDUARDO. Canine fetus immune system at late development. ANIMAL REPRODUCTION, v. 16, n. 2, p. 328-331, APR-JUN 2019. (15/17897-9)

SANTOS ROBALLO, KELLY CRISTINE; DA SILVEIRA, JULIANO COELHO; BRESSAN, FABIANA FERNANDES; DE SOUZA, ALINE FERNANDA; PEREIRA, VITORIA MATTOS; PINZON PORRAS, JORGE ELIECER; ROS, FELIPE AUGUSTO; PULZ, LIDIA HILDEBRAND; STREFEZZI, RICARDO DE FRANCISCO; MARTINS, DANIELE DOS SANTOS; et al. Neurons-derived extracellular vesicles promote neural differentiation of ADSCs: a model to prevent peripheral nerve degeneration. SCIENTIFIC REPORTS, v. 9, AUG 1 2019. (15/17897-9, 14/22887-0, 15/21829-9, 13/08135-2)

ROSANE MAZZARELLA; TAISMARA K. GARNICA; KELLY C.S. ROBALLO; CARLOS E. AMBRÓSIO. Células-tronco derivadas do epitélio olfatório: perspectivas terapêuticas na medicina veterinária. Pesquisa Veterinária Brasileira, v. 36, n. 8, p. 787-792, 2016-08-00. (15/17897-9)

GOMES, INGRID S.; DE OLIVEIRA, VANESSA C.; PINHEIRO, ALESSANDRA O.; ROBALLO, KELLY C. S.; DE ARAUJO, GIULIANO S. M.; VERONEZI, JESSICA C.; MARTINS, DANIELE S.; AMBROSIO, CARLOS EDUARDO. Bone marrow stem cell applied in the canine veterinary clinics. Pesquisa Veterinária Brasileira, v. 37, n. 10, p. 7-pg., 2017-10-01. (15/17897-9)

JULIANA BARBOSA CASALS; NAIRA CAROLINE GODOY PIERI; KELLY CRISTINE SANTOS ROBALLO; FABIANA FERNANDES BRESSAN; PHELIPE OLIVEIRA FAVARON; DANIELE DOS SANTOS MARTINS; CARLOS EDUARDO AMBRÓSIO. Pluripotent stem cells proliferation is associated with placentation in dogs. Animal Reproduction, v. 17, n. 3, 2020-08-17. (13/09392-9, 15/17897-9)

GOMES, INGRID S.; DE OLIVEIRA, VANESSA C.; PINHEIRO, ALESSANDRA O.; ROBALLO, KELLY C. S.; DE ARAUJO, GIULIANO S. M.; VERONEZI, JESSICA C.; MARTINS, DANIELE S.; AMBROSIO, CARLOS EDUARDO. Bone marrow stem cell applied in the canine veterinary clinics. Pesquisa Veterinária Brasileira, v. 37, n. 10, p. 1139-1145, OCT 2017. (15/17897-9)

ROBALLO, KELLY C. S.; GONCALVES, NATALIA J. N.; PIERI, NAIRA C. G.; SOUZA, ALINE F.; ANDRADE, ANDRE F. C.; AMBROSIO, CARLOS E.. Regulation of neural stem cells by choroid plexus cells population. Neuroscience Letters, v. 626, p. 35-41, JUL 28 2016. (15/17897-9, 13/07673-0)

CASALS, JULIANA BARBOSA; GODOY PIERI, NAIRA CAROLINE; SANTOS ROBALLO, KELLY CRISTINE; BRESSAN, FABIANA FERNANDES; FAVARON, PHELIPE OLIVEIRA; MARTINS, DANIELE DOS SANTOS; AMBROSIO, CARLOS EDUARDO. Pluripotent stem cells proliferation is associated with placentation in dogs. ANIMAL REPRODUCTION, v. 17, n. 3, p. 10-pg., 2020-01-01. (15/17897-9, 13/09392-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ROBALLO, Kelly Cristine Santos. Exosome communication in the transdifferentiation of stem cells in co-culture with neuronal cells. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Zootecnica e Engenharia de Alimentos (FZE/BT) Pirassununga.