Study of the effect of short-chain fatty acids on the tissue regeneration process during experimental colitis

• Grant number: 20/02312-3
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: March 01, 2020
• End date: February 28, 2025
• Field of knowledge: Biological Sciences - Immunology - Cellular Immunology
• Principal Investigator: Marco Aurélio Ramirez Vinolo
• Grantee: Arilson Bernardo dos Santos Pereira Gomes
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 18/15313-8 - Investigation of the molecular mechanisms involved in the interaction between microbiota-derived metabolites and host cells during inflammation, AP.JP2
• Associated scholarship(s): 23/00564-3 - Understanding the mechanisms by which inulin modulate the intestinal microbiota and contribute to epithelial regeneration in the colon, BE.EP.DD

Abstract

Colitis is a major Inflammatory Bowel Disease (IBD) and represent an important public health problem. The intestinal microbiota plays an important role in maintaining intestinal homeostasis and contributes to communication with host cells. During epithelial regeneration, Intestinal Stem Cells (ISCs), present in crypts, give rise to precursor cells. It is not yet well understood, how ISCs respond to various signals under pathological conditions. The products of bacterial metabolism, Short-Chain Fatty Acids (SCFA), they are generated during fermentation of non-digestible carbohydrates in the intestine and constitute an important relationship in microbiota-host communication. Studies show that these bioactive agents regulate the host's metabolism and immunity, however, the molecular mechanisms that are involved have not been fully defined. How ISC has relevant participation in the intestinal mucosa and a wide range of interactions with a microbiota, the objective of this project is to assess the role of SCFA and their FFAR2 receptor in the modulation of intestinal epithelial regeneration in the model of colitis induced by 2.5% sodium dextran sulfate (DSS). For this, we will use mice wild-type and knockout for FFAR2, both whole body and tissue-specific, and we will analyze the role of this recptor and the SCFA in the modulation of intestinal epithelial regeneration. In this study, we hope to contribute to the understanding of mechanisms and interaction between host microbiota and identification of a possible therapeutic target. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RODRIGUES, PATRICIA BRITO; DOS SANTOS PEREIRA GOMES, ARILSON BERNARDO; GENARO, LIVIA MOREIRA; PASCOAL, LIVIA BITENCOURT; DUARTE DE SOUZA, ANA PAULA; LEAL, RAQUEL FRANCO; RAMIREZ VINOLO, MARCO AURELIO; GUEST, PC. Intestinal Microbiota in the SARS-CoV-2 Infection: What Is Known?. IDENTIFCATION OF BIOMARKERS, NEW TREATMENTS, AND VACCINES FOR COVID-19, v. 1327, p. 14-pg., 2021-01-01. (20/02312-3, 20/04583-4, 19/14342-7)

PASCOAL, LIVIA BITENCOURT; RODRIGUES, PATRICIA BRITO; GENARO, LIVIA MOREIRA; DOS SANTOS PEREIRA GOMES, ARILSON BERNARDO; TOLEDO-TEIXEIRA, DANIEL AUGUSTO; PARISE, PIERINA LORENCINI; BISPO-DOS-SANTOS, KARINA; SIMEONI, CAMILA LOPES; GUIMARAES, PAULA VERI; BUSCARATTI, LUCAS ILDEFONSO; et al. Microbiota-derived short-chain fatty acids do not interfere with SARS-CoV-2 infection of human colonic samples. GUT MICROBES, v. 13, n. 1, p. 1-9, JAN 1 2021. (20/04558-0, 20/04583-4, 20/02448-2, 20/02312-3, 19/14342-7, 20/04746-0, 17/26908-0, 20/04579-7, 20/04919-2, 19/06372-3, 13/07607-8, 20/02159-0)

CORREA, RENAN OLIVEIRA; CASTRO, POLLYANA RIBEIRO; FACHI, JOSE LUIS; NIRELLO, VINICIUS DIAS; EL-SAHHAR, SALMA; IMADA, SHINYA; PEREIRA, GABRIEL VASCONCELOS; PRAL, LAIS PASSARIELLO; PEREIRA ARAUJO, NATHALIA VITORIA; FERNANDES, MARIANE FONT; et al. Inulin diet uncovers complex diet-microbiota-immune cell interactions remodeling the gut epithelium. MICROBIOME, v. 11, n. 1, p. 25-pg., 2023-04-26. (16/23142-3, 21/06572-2, 19/06372-3, 19/16113-5, 19/02640-3, 18/15313-8, 20/02919-5, 17/16280-3, 20/14071-0, 21/00393-9, 17/06577-9, 20/13689-0, 21/05269-4, 20/02312-3, 18/10165-0, 20/00311-0)