Bacterial viability after antimicrobial photodynamic therapy with curcumin on multiresistant Staphylococcus aureus

Autor(es)

Freitas, Mirian A. A. ^[1] ; Pereira, Andre H. C. ^[1] ; Pinto, Juliana G. ^[1] ; Casas, Adriana ^[2] ; Ferreira-Strixino, Juliana ^[1]

Afiliação do(s) autor(es)

^[1] Univ Vale Paraiba, R&DI, Photodynam Therapy Lab, Univap, Shishima Hifumi Ave 2911, BR-12244000 Sao Jose Dos Campos, SP - Brazil

^[2] Univ Buenos Aires, CONICET, Htal Clin Gral Jose de San Martin, CIPYP, Buenos Aires, DF - Argentina

• Tipo de documento: Artigo Científico
• Fonte: FUTURE MICROBIOLOGY; v. 14, n. 9, p. 739-748, JUL 4 2019.
• Citações Web of Science: 3

Resumo

Staphylococcus aureus are multiresistant pathogens that causes superficial and systemic infections. Antimicrobial photodynamic therapy (APDT) is an alternative in the treatment of diseases caused by these bacteria. Aim: In this study the APDT response on growth, viability, formation of reactive oxygen species and adhesion of methicillin-sensitive strains of Staphylococcus aureus, strains of methicillin-resistant S. aureusand American-type culture collection (ATCC) of S. aureus were evaluated in vitro, after incubation with curcumin for 20min, and irradiated with LED. Materials \& methods: Bacterial growth was assessed by the number of colony-forming units, viability and adhesion were evaluated by confocal microscopy and ROS quantification was performed by fluorimetry. Results: Was observed increase in the production of ROS in APDT groups, besides a decrease in the 4 log growth and loss of the bacterial adhesion. Conclusion: APDT with Curcumin may be an interesting therapeutic alternative, due to its in vitro response, in the control multiresistant clinical S. aureus strains. (AU)

• Processo FAPESP: 16/12211-4 - Estudo do efeito genotóxico, do potencial de membrana e das vias de morte celular da curcumina na terapia fotodinâmica em promastigota e amastigota de L. major e L. braziliensis: estudo in vitro
• Beneficiário: Juliana Ferreira Strixino
• Modalidade de apoio: Auxílio à Pesquisa - Regular