A proteotranscriptomic study of silk-producing glands from the orb-weaving spiders

Autor(es):Mostrar menos -

Aparecido dos Santos-Pinto, Jose Roberto ^[1] ; Esteves, Franciele Grego ^[1] ; Sialana, Fernando J. ^[2] ; Ferro, Milene ^[1] ; Smidak, Roman ^[2] ; Rares, Lucaciu Calin ^[3] ; Nussbaumer, Thomas ^[3] ; Rattei, Thomas ^[3] ; Bilban, Martin ^[4] ; Bacci Junior, Mauricio ^[1] ; Palma, Mario Sergio ^[1] ; Luebec, Gert ^[5]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Inst Biosci Rio Claro, Dept Biol, Ctr Study Social Insects, BR-13500 Rio Claro, SP - Brazil

^[2] Univ Vienna, Dept Pharmaceut Chem, A-1090 Vienna - Austria

^[3] Univ Vienna, Dept Microbiol & Ecosyst Sci, Div Computat Syst Biol, A-1090 Vienna - Austria

^[4] Med Univ Vienna, Dept Lab Med & Core Facil Genom, A-1090 Vienna - Austria

^[5] Paracelsus Med Univ, A-5020 Salzburg - Austria

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR OMICS; v. 15, n. 4, p. 256-270, AUG 1 2019.
• Citações Web of Science: 0

Resumo

Orb-weaving spiders can produce different silk fibers, which constitute outstanding materials characterized by their high strength and elasticity. Researchers have tried to reproduce the fibers of these proteins synthetically and/or by using recombinant DNA technology, but only a few of the natural physicochemical and biophysical properties have been obtained to date. Female orb-web-spiders present seven silk-glands, which synthesize the spidroins and a series of other proteins, which interact with the spidroins, resulting in silk fibers with notable physicochemical properties. Despite the recognized importance of the silk-glands for understanding how the fibers are produced and processed, the investigation of these glands is at a nascent stage. In the current study we present the assembled transcriptome of silk-producing glands from the orb-weaving spider Nephila clavipes, as well as develop a large-scale proteomic approach for in-depth analyses of silk-producing glands. The present investigation revealed an extensive repertoire of hitherto undescribed proteins involved in silk secretion and processing, such as prevention of degradation during the silk spinning process, transportation, protection against proteolytic autolysis and against oxidative stress, molecular folding and stabilization, and post-translational modifications. Comparative phylogenomic-level evolutionary analyses revealed orthologous genes among three groups of silk-producing organisms - (i) Araneomorphae spiders, (ii) Mygalomorphae spiders, and (iii) silk-producing insects. A common orthologous gene, which was annotated as silk gland factor-3 is present among all species analysed. This protein belongs to a transcription factor family, that is important and related to the development of the silk apparatus synthesis in the silk glands of silk-producing arthropods. (AU)

• Processo FAPESP: 16/16212-5 - Proteopeptídeos naturais da fauna, flora e microbiota brasileira como potenciais modelos para o desenvolvimento racional de novos fármacos de uso terapêutico: isolamento, elucidação estrutural, síntese química e ensaios de atividade funcional
• Beneficiário: Mario Sergio Palma
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 16/10430-0 - Uma visão proteotranscriptomica sobre o papel das glândulas de seda de aranhas durante o processo de fiação da seda
• Beneficiário: José Roberto Aparecido dos Santos-Pinto
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 13/26451-9 - Bioprospecção e Análise Estrutural das Proteínas da Seda de Artrópodes por uma Abordagem Proteômica Utilizando um Sistema nanoLC-ESI-CID/ETD
• Beneficiário: José Roberto Aparecido dos Santos-Pinto
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/10373-0 - Perfilagem peptidômica e caracterização estrutural-funcional das vesículas lipídicas presentes na teia da aranha Nephila clavipes
• Beneficiário: Franciele Grego Esteves
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/14220-8 - Caracterização proteometabolômica dos componentes da teia da aranha Nephila clavipes utilizados na estratégia de captura de presas
• Beneficiário: Franciele Grego Esteves
• Modalidade de apoio: Bolsas no Brasil - Mestrado