Genome Sequences of a Plant Beneficial Synthetic Bacterial Community Reveal Genetic Features for Successful Plant Colonization

Autor(es)

Correa de Souza, Rafael Soares ^{[1, 2, 3]} ; Leite Armanhi, Jaderson Silveira ^{[1, 2, 3]} ; Damasceno, Natalia de Brito ^{[1, 2, 3]} ; Imperial, Juan ^{[4, 5]} ; Arruda, Paulo ^{[1, 2, 3]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Genet & Evolucao, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, GCCRC, Campinas, SP - Brazil

^[4] Univ Politecn Madrid, Inst Nacl Invest & Tecnol Agr & Alimentaria INIA, Ctr Biotecnol & Genom Plantas, Campus Montegancedo UPM, Madrid - Spain

^[5] CSIC, Inst Ciencias Agr, Madrid - Spain

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN MICROBIOLOGY; v. 10, AUG 13 2019.
• Citações Web of Science: 0

Resumo

Despite the availability of data on the functional and phylogenetic diversity of plant-associated microbiota, the molecular mechanisms governing the successful establishment of plant bacterial communities remain mostly elusive. To investigate bacterial traits associated with successful colonization of plants, we sequenced the genome of 26 bacteria of a synthetic microbial community (SynCom), 12 of which displayed robust and 14 displayed non-robust colonization lifestyles when inoculated in maize plants. We examined the colonization profile of individual bacteria in inoculated plants and inspected their genomes for traits correlated to the colonization lifestyle. Comparative genomic analysis between robust and non-robust bacteria revealed that commonly investigated plant growth-promoting features such as auxin production, nitrogen (N) fixation, phosphate acquisition, and ACC deaminase are not deterministic for robust colonization. Functions related to carbon (C) and N acquisition, including transporters of carbohydrates and amino acids, and kinases involved in signaling mechanisms associated with C and N uptake, were enriched in robust colonizers. While enrichment of carbohydrate transporters was linked to a wide range of metabolites, amino acid transporters were primarily related to the uptake of branched-chain amino acids. Our findings identify diversification of nutrient uptake phenotypes in bacteria as determinants for successful bacterial colonization of plants. (AU)

• Processo FAPESP: 18/19100-9 - Desenvolvendo uma comunidade microbiana sintética para promoção de tolerância à seca em milho
• Beneficiário: Rafael Soares Correa de Souza
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/04322-0 - Identificação de relações microrganismo/microrganismo e microrganismo/planta à promoção do crescimento vegetal a partir de uma coleção de bactérias representativa do microbioma da cana-de-açúcar
• Beneficiário: Jaderson Silveira Leite Armanhi
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 16/23218-0 - Centro de Pesquisa em Genômica Aplicada às Mudanças Climáticas
• Beneficiário: Edi Lúcia Sartorato
• Modalidade de apoio: Auxílio à Pesquisa - Programa Centros de Pesquisa em Engenharia
• Processo FAPESP: 16/23425-5 - Mapeamento da colonização de plantas de milho e de soja por uma comunidade microbiana sintética oriunda do microbioma da cana-de-açúcar
• Beneficiário: Natália de Brito Damasceno
• Modalidade de apoio: Bolsas no Brasil - Mestrado