Polymerized laminin incorporation into alginate-based microcapsules reduces pericapsular overgrowth and inflammation

Autor(es)

Leal-Lopes, Camila ^{[1, 2]} ; Grazioli, Gisella ^[2] ; Mares-Guia, Thiago R. ^[2] ; Coelho-Sampaio, Tatiana ^[3] ; Sogayar, Mari Cleide ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Nucleo Terapia Celular Mol NUCEL, Dept Clin Med, Fac Med, Sao Paulo - Brazil

^[3] Univ Fed Rio de Janeiro, Inst Ciencias Biomed, Rio De Janeiro - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE; v. 13, n. 10 AUG 2019.
• Citações Web of Science: 0

Resumo

Cell encapsulation coats cells with an artificial membrane to preserve their physical and functional integrity. Different approaches try to develop more functional and biocompatible materials to avoid cell loss after transplantation due to inflammatory reaction, one of the main causes for graft failure. In this study, the LN-Biodritin biomaterial, based on alginate, chondroitin sulfate, and laminin, previously developed by our group, was further improved by replacing laminin by polylaminin, an artificial laminin polymer with anti-inflammatory properties, generating the new biomaterial polyLN-Biodritin. Capsules containing polylaminin are stable, do not induce macrophage activation in vitro, and are also able to prevent macrophage activation by encapsulated human pancreatic islets in vitro, preserving their glucose-stimulated insulin secretion potential. In addition, when empty capsules containing polylaminin were implanted into immunocompetent mice, the inflammatory response towards the implant was attenuated, when compared with capsules without polylaminin. The results indicate that polylaminin incorporation leads to lower levels of pericapsular growth on the capsules surface, lower infiltration of cells into the peritoneal cavity, and lower production of proinflammatory cytokines, both at the implant site (interleukin-12p70 (IL-12p70), tumor necrosis factor-alpha (TNF-alpha), monocyte chemotactic protein-1 (MCP-1), and interferon-gamma (IFN-gamma)) and systemically (IL-12p70 and TNF-alpha). Therefore, polylaminin incorporation into the microcapsules polymer attenuates the host posttransplantation immune response against implanted microcapsules, being likely to favor maintenance of engrafted encapsulated cells. (AU)

• Processo FAPESP: 16/05311-2 - Medicina regenerativa visando à terapia de doenças crônico-degenerativas (câncer e diabetes)
• Beneficiário: Mari Cleide Sogayar
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/00664-6 - Terapias alternativas para o DM1: geração de biomateriais inovadores para microencapsulamento de ilhotas e agregados celulares resultantes da diferenciação de mESCs e estudo funcional de genes diferencialmente expressos durante este processo
• Beneficiário: Camila Leal Lopes da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto