Estrogen receptor gene is associated with dental fluorosis in Brazilian children

Autor(es)

Dalledone, M. ^[1] ; Cunha, A. S. ^[2] ; Ramazzotto, L. A. ^{[2, 3]} ; Pecharki, G. D. ^[4] ; Nelson-Filho, P. ^[2] ; Scariot, R. ^[1] ; Trevilatto, P. C. ^[5] ; Vieira, A. R. ^[6] ; Kuchler, Erika C. ^{[2, 1]} ; Brancher, Joao A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Positivo, Sch Hlth & Biol Sci, Rua Prof Pedro Viriato 5Parigot de Souza 300, BR-80740050 Curitiba, Parana - Brazil

^[2] Univ Sao Paulo, Dept Pediat Dent, Sch Dent Ribeirao Preto, Ave Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil

^[3] Univ Fed Sao Carlos, Biotechnol Grad, Sao Carlos, SP - Brazil

^[4] Univ Fed Parana, Dept Community Hlth, Curitiba, Parana - Brazil

^[5] Pontificia Univ Catolica Parana, Sch Life Sci, Curitiba, Parana - Brazil

^[6] Univ Pittsburgh, Sch Dent Med, Dept Oral Biol, Pittsburgh, PA 15260 - USA

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL ORAL INVESTIGATIONS; v. 23, n. 9, p. 3565-3570, SEP 2019.
• Citações Web of Science: 0

Resumo

Objectives The aim of this study was investigate the association between genetic polymorphisms in ESR1, ESR2, and ESRRB and dental fluorosis (DF) in a well-characterized sample of children from Curitiba, Brazil. Material and methods From a representative sample of 538 children, 12-year-old were evaluated. DF was assessed in erupted permanent teeth by the Dean's index modified. Fourteen polymorphisms were selected in intronic and intergenic regions of ESR1, ESR2, and ESRRB and genotyped in genomic DNA source from saliva using TaqMan chemistry and end-point analysis. Allele and genotype distributions between DF and DF free groups were analyzed using the Epi Info 7.2. Chi-square or Fisher's exact tests at a level of significance of 5% and odds ratios calculations with 95% confidence intervals were used to determine the statistical associations. Results Among 538 children, 147 were DF and 391 were DF free. Genotype distribution for the polymorphism rs12154178 in ESR1 was different between the two groups (p = 0.037; OR = 0.91; CI = 0.67-1.22). The dominant model analysis (AA+AC vs. CC) demonstrated that CC is a protective factor for DF (p = 0.038; OR = 0.51, 0.27-0.97 95% CI). We did not find differences in frequency distributions in the other evaluated polymorphisms. Conclusion This study provides evidence that ESR1 is associated with DF. (AU)

• Processo FAPESP: 15/06866-5 - Avaliação do papel do estrógeno no desenvolvimento dentofacial
• Beneficiário: Erika Calvano Kuchler
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores