Tissue Constructs with Human Adipose-Derived Mesenchymal Stem Cells to Treat Bone Defects in Rats

Autor(es):Mostrar menos -

Caetano, Guilherme ^{[1, 2]} ; Wang, Weiguang ^[3] ; Murashima, Adriana ^[4] ; Passarini, Jr., Jose Roberto ^[2] ; Bagne, Leonardo ^[2] ; Leite, Marcel ^[1] ; Hyppolito, Miguel ^[4] ; Al-Deyab, Salem ^[5] ; El-Newehy, Mohamed ^{[5, 6]} ; Bartolo, Paulo ^[3] ; Cipriani Frade, Marco Andrey ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Internal Med, BR-14040900 Ribeirao Preto, SP - Brazil

^[2] Univ Ctr Herminio Ometto Fdn, Grad Program Biomed Sci, BR-13607339 Araras, SP - Brazil

^[3] Univ Manchester, Sch Mech Aerosp & Civil Engn, Manchester M13 9PL, Lancs - England

^[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Ophthalmol Otolaryngol & Head & Neck Surg, BR-14040900 Ribeirao Preto, SP - Brazil

^[5] King Saud Univ, Coll Sci, Dept Chem, Riyadh 11451 - Saudi Arabia

^[6] Tanta Univ, Fac Sci, Dept Chem, Tanta 31527 - Egypt

• Tipo de documento: Artigo Científico
• Fonte: MATERIALS; v. 12, n. 14 JUL 2 2019.
• Citações Web of Science: 2

Resumo

The use of porous scaffolds created by additive manufacturing is considered a viable approach for the regeneration of critical-size bone defects. This paper investigates the xenotransplantation of polycaprolactone (PCL) tissue constructs seeded with differentiated and undifferentiated human adipose-derived mesenchymal stem cells (hADSCs) to treat calvarial critical-sized defect in Wistar rats. PCL scaffolds without cells were also considered. In vitro and in vivo biological evaluations were performed to assess the feasibility of these different approaches. In the case of cell seeded scaffolds, it was possible to observe the presence of hADSCs in the rat tissue contributing directly (osteoblasts) and indirectly (stimulation by paracrine factors) to tissue formation, organization and mineralization. The presence of bone morphogenetic protein-2 (BMP-2) in the rat tissue treated with cell-seeded PCL scaffolds suggests that the paracrine factors of undifferentiated hADSC cells could stimulate BMP-2 production by surrounding cells, leading to osteogenesis. Moreover, BMP-2 acts synergistically with growth factors to induce angiogenesis, leading to higher numbers of blood vessels in the groups containing undifferentiated and differentiated hADSCs. (AU)

• Processo FAPESP: 13/20554-0 - Osteodiferenciação das células-tronco mesenquimais humanas associadas às proteínas do látex natural da seringueira Hevea Brasiliensis sobre scaffold de policaprolactona e ácido polilático (PCL/PLA) para xenoenxertia óssea
• Beneficiário: Marco Andrey Cipriani Frade
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/09055-2 - Osteodiferenciação das células-tronco mesenquimais humanas associadas às proteínas do látex natural da seringueira Hevea brasiliensis sobre scaffold de policaprolactona e ácido polilático (PCL/PLA) para xenoenxertia óssea
• Beneficiário: Guilherme Ferreira Caetano
• Modalidade de apoio: Bolsas no Brasil - Doutorado