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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Tissue Constructs with Human Adipose-Derived Mesenchymal Stem Cells to Treat Bone Defects in Rats

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Caetano, Guilherme [1, 2] ; Wang, Weiguang [3] ; Murashima, Adriana [4] ; Passarini, Jr., Jose Roberto [2] ; Bagne, Leonardo [2] ; Leite, Marcel [1] ; Hyppolito, Miguel [4] ; Al-Deyab, Salem [5] ; El-Newehy, Mohamed [5, 6] ; Bartolo, Paulo [3] ; Cipriani Frade, Marco Andrey [1]
Total Authors: 11
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Internal Med, BR-14040900 Ribeirao Preto, SP - Brazil
[2] Univ Ctr Herminio Ometto Fdn, Grad Program Biomed Sci, BR-13607339 Araras, SP - Brazil
[3] Univ Manchester, Sch Mech Aerosp & Civil Engn, Manchester M13 9PL, Lancs - England
[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Ophthalmol Otolaryngol & Head & Neck Surg, BR-14040900 Ribeirao Preto, SP - Brazil
[5] King Saud Univ, Coll Sci, Dept Chem, Riyadh 11451 - Saudi Arabia
[6] Tanta Univ, Fac Sci, Dept Chem, Tanta 31527 - Egypt
Total Affiliations: 6
Document type: Journal article
Source: MATERIALS; v. 12, n. 14 JUL 2 2019.
Web of Science Citations: 2

The use of porous scaffolds created by additive manufacturing is considered a viable approach for the regeneration of critical-size bone defects. This paper investigates the xenotransplantation of polycaprolactone (PCL) tissue constructs seeded with differentiated and undifferentiated human adipose-derived mesenchymal stem cells (hADSCs) to treat calvarial critical-sized defect in Wistar rats. PCL scaffolds without cells were also considered. In vitro and in vivo biological evaluations were performed to assess the feasibility of these different approaches. In the case of cell seeded scaffolds, it was possible to observe the presence of hADSCs in the rat tissue contributing directly (osteoblasts) and indirectly (stimulation by paracrine factors) to tissue formation, organization and mineralization. The presence of bone morphogenetic protein-2 (BMP-2) in the rat tissue treated with cell-seeded PCL scaffolds suggests that the paracrine factors of undifferentiated hADSC cells could stimulate BMP-2 production by surrounding cells, leading to osteogenesis. Moreover, BMP-2 acts synergistically with growth factors to induce angiogenesis, leading to higher numbers of blood vessels in the groups containing undifferentiated and differentiated hADSCs. (AU)

FAPESP's process: 13/20554-0 - Osteo-differentiation of human mesenchymal stem cells attached to natural latex proteins from the rubber tree Hevea brasiliensis on polycaprolactone and polylactic acid scaffold (PCL/PLA) to bone xenografting
Grantee:Marco Andrey Cipriani Frade
Support Opportunities: Regular Research Grants
FAPESP's process: 13/09055-2 - Osteo-differentiation of human mesenchymal stem cells attached to natural latex proteins from the rubber tree Hevea brasiliensis on scaffold of polycaprolactone and polylactic acid (PCL/PLA) to bone xenografting
Grantee:Guilherme Ferreira Caetano
Support Opportunities: Scholarships in Brazil - Doctorate