Antileishmanial activity and immunomodulatory effect of secosubamolide, a butanolide isolated from Nectandra oppositifolia (Lauraceae)

Autor(es)

da Costa-Silva, Thais A. ^[1] ; Alves Conserva, Geanne A. ^[1] ; Galisteo, Jr., Andres J. ^[2] ; Tempone, Andre G. ^[3] ; Lago, Joao Henrique G. ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ ABC UFBAC, Ctr Nat Sci & Humanities, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Trop Med, Sao Paulo, SP - Brazil

^[3] Adolfo Lutz Inst, Ctr Parasitol & Mycol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 25, AUG 19 2019.
• Citações Web of Science: 0

Resumo

Background: Visceral leishmaniasis is a complex neglected tropical disease caused by Leishmania donovani complex. Its current treatment reveals strong limitations, especially high toxicity. In this context, natural products are important sources of new drug alternatives for VL therapy. Therefore, the antileishmanial and immunomodulatory activity of compounds isolated from Nectandra oppositifolia (Lauraceae) was investigated herein. Methods: The n-hexane extract from twigs of N. oppositifolia were subjected to HPLC/HRESIMS and bioactivity-guided fractionation to afford compounds 1 and 2 which were evaluated in vitro against Leishmania (L.) infantum chagasi and NCTC cells. Results: The n-hexane extract displayed activity against L. (L.) infantum chagasi and afforded isolinderanolide E (1) and secosubamolide A (2), which were effective against L. (L.) infantum chagasi promastigotes, with IC50 values of 57.9 and 24.9 mu M, respectively. Compound 2 was effective against amastigotes (IC50 = 10.5 mu M) and displayed moderate mammalian cytotoxicity (CC50 = 42 mu M). The immunomodulatory studies of compound 2 suggested an anti-inflammatory activity, with suppression of IL-6, IL-10, TNF with lack of nitric oxide. Conclusion: This study showed the antileishmanial activity of compounds 1 and 2 isolated from N. oppositifolia. Furthermore, compound 2 demonstrated an antileishmanial activity towards amastigotes associated to an immunomodulatory effect. (AU)

• Processo FAPESP: 16/20633-6 - METABOLITOS BIOATIVOS DE Nectandra oppositifolia Ness & Mart. (LAURACEAE) - CARACTERIZAÇÃO MOLECULAR, AVALIAÇÃO DO POTENCIAL ANTIPARASITÁRIO in vitro E in vivo E DETERMINAÇÃO DOS MECANISMOS DE AÇÃO
• Beneficiário: Geanne Alexsandra Alves Conserva
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/10279-6 - Seleção e Otimização de Novos Candidatos a Fármacos para Leishmaniose e Doença de Chagas
• Beneficiário: André Gustavo Tempone Cardoso
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/07885-1 - Biomoléculas oriundas de espécies vegetais de áreas remanescentes da Mata Atlântica e do Cerrado para tratamento de doenças tropicais negligenciadas - aspectos químicos e farmacológicos
• Beneficiário: João Henrique Ghilardi Lago
• Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Regular