Laccase stabilized on beta-D-glucan films on the surface of carbon black/gold nanoparticles: A new platform for electrochemical biosensing

Autor(es)

Mattos, Gabriel J. ^[1] ; Moraes, Jaqueline T. ^[1] ; Barbosa, Eduardo C. M. ^[2] ; Camargo, Pedro H. C. ^[2] ; Dekker, Robert F. H. ^[3] ; Barbosa-Dekker, Aneli M. ^[1] ; Sartori, Elen R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Londrina, Dept Quim, Ctr Ciencias Exatas, Rodovia Celso Garcia Cid, PR 445, Km 380, BR-86057970 Londrina, PR - Brazil

^[2] Univ Sao Paulo, Dept Quim Fundamental, Inst Quim, Av Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil

^[3] Univ Tecnol Fed Parana, Programa Posgrad Engn Ambiental, Campus Londrina, BR-86036370 Londrina, PR - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOELECTROCHEMISTRY; v. 129, p. 116-123, OCT 2019.
• Citações Web of Science: 1

Resumo

In this study, (1 -> 3)(1 -> 6)-beta-D-glucan (botryosphaeran) from Botryosphaeria rhodina MAMB-05 was used, for the first time, to immobilize laccase on a carbon black paste electrode modified with gold nanoparticles. The physicochemical characterization of the proposed laccase-biosensor was performed using transmission electron microscopy and electrochemical impedance spectroscopy. The performance of this novel bio-device was evaluated by choosing hydroquinone as a typical model of a phenolic compound. For hydroquinone determination, experimental variables such as enzyme concentration, pH and operational parameters of the electroanalytical technique were optimized. From square-wave voltammograms, a linear dependence between the cathodic current peak and the hydroquinone concentration was observed within the range 2.00-56.5 mu mol L-1, with a theoretical detection limit of 0.474 mu mol L-1. The proposed method was successfully applied to determine hydroquinone in dermatological cream, and samples from biological and environmental niches. The proposed biosensor device presented good selectivity in the presence of uric acid, various inorganic ions, as well as other phenolic compounds, demonstrating the potential application of this biosensing platform in complex matrices. Operational and analytical stability of the laccase biosensor were evaluated, and demonstrated good intra-day (SD = 0.3%) and inter-day (SD = 3.4%) repeatability and long storage stability (SD = 4.9%). (C) 2019 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 15/26308-7 - Otimização das propriedades físico-químicas de materiais nano-estruturados e suas aplicações em reconhecimento molecular, catálise e conversão/armazenamento de energia
• Beneficiário: Roberto Manuel Torresi
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/21366-9 - Materiais híbridos contendo nanopartículas metálicas para aplicações catalíticas
• Beneficiário: Pedro Henrique Cury Camargo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/11452-5 - Materiais híbridos compostos por óxidos e nanopartículas metálicas para aplicações catalíticas e fotocatalíticas
• Beneficiário: Eduardo César Melo Barbosa
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto