Beneficial Effects of Ethyl-Cyanoacrylate Coating Against Candida Albicans Biofilm Formation

Autor(es)

Flora Freitas Fernandes Távora ^[1] ; Ana Paula Chappuis Chocano ^[2] ; Denise Gusmão de Oliveira ^[3] ; Jefferson Ricardo Pereira ^[4] ; Ricardo Sergio Almeida ^[5] ; Karin Hermana Neppelenbroek ^[6] ; Vinícius Carvalho Porto ^[7]

Afiliação do(s) autor(es)

^[1] USC - Universidade do Sagrado Coração. Department of Implantology and Prosthodontics - Brasil

^[2] USP - Universidade de São Paulo. Department of Prosthodontics and Periodontics - Brasil

^[3] UniNorte - Laureate International Universities - Brasil

^[4] UNISUL - Universidade do Sul de Santa Catarina. Program of Health Science - Brasil

^[5] UEL - Universidade Estadual de Londrina. Department of Microbiology - Brasil

^[6] USP - Universidade de São Paulo. Department of Prosthodontics and Periodontics - Brasil

^[7] USP - Universidade de São Paulo. Department of Prosthodontics and Periodontics - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 30, n. 3, p. 266-271, 2019-06-03.

Resumo

Abstract The aim of this study was to verify whether modifications made in a hard chairside reline resin by an ethyl-cyanoacrylate adhesive, ECA (Super Bonder®, Loctite, Itapevi, SP, Brazil) would be able to inhibit or reduce Candida albicans biofilm formation on its surface, comparing to a commercial surface sealant (BisCover®, Bisco, Schaumburg, USA). Reline resin specimens were fabricated and randomly divided into 6 groups (n=8): CG (control group), no surface treatment; ECA1, ECA coating on the surface before sterilization; ECA2, ECA coating after sterilization; ECA3, ECA incorporated in the resin bulk; DPE1, BisCover® coating before sterilization; DPE2, BisCover® coating after sterilization. Specimens were inoculated with C. albicans SC5314 (1x107 cells/mL) and incubated for 24 h. Then, the biofilm were stained with LIVE/DEAD® BaclightTM L7007 Kit and analyzed by Confocal Laser Scanning Microscopy. The images were evaluated by bioImageL® v.2.0 software and total biovolume (µm3), viable cells (%), and covered area (%) were calculated. Data were statistically analyzed by Kruskal-Wallis and Dunn tests (p<0.05). Results showed that ECA-coated groups presented better results, reducing C. albicans biofilm formation. Acquired images revealed that these groups (ECA1 and ECA2) presented a reduced number of cells, mostly in yeast form (less pathogenic), while the other groups presented higher number of cells, mostly in hyphae form (more pathogenic). Based on these findings, a beneficial effect of Super Bonder® coating reline resins surface could be demonstrated, suggesting a promising way to prevent fungal biofilm formation on dentures. (AU)

• Processo FAPESP: 14/09426-3 - Efeito de selante tissular à base de veneno de cobra e cianoacrilatos aplicados em resina acrílica na quantidade e atividade das células de C. albicans, citotoxicidade sobre fibroblastos gengivais (fase I) e ensaio de degradação das superfícies (fase II)
• Beneficiário: Vinicíus Carvalho Porto
• Modalidade de apoio: Auxílio à Pesquisa - Regular