Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Beneficial Effects of Ethyl-Cyanoacrylate Coating Against Candida Albicans Biofilm Formation

Full text
Author(s):
Flora Freitas Fernandes Távora [1] ; Ana Paula Chappuis Chocano [2] ; Denise Gusmão de Oliveira [3] ; Jefferson Ricardo Pereira [4] ; Ricardo Sergio Almeida [5] ; Karin Hermana Neppelenbroek [6] ; Vinícius Carvalho Porto [7]
Total Authors: 7
Affiliation:
[1] USC - Universidade do Sagrado Coração. Department of Implantology and Prosthodontics - Brasil
[2] USP - Universidade de São Paulo. Department of Prosthodontics and Periodontics - Brasil
[3] UniNorte - Laureate International Universities - Brasil
[4] UNISUL - Universidade do Sul de Santa Catarina. Program of Health Science - Brasil
[5] UEL - Universidade Estadual de Londrina. Department of Microbiology - Brasil
[6] USP - Universidade de São Paulo. Department of Prosthodontics and Periodontics - Brasil
[7] USP - Universidade de São Paulo. Department of Prosthodontics and Periodontics - Brasil
Total Affiliations: 7
Document type: Journal article
Source: Brazilian Dental Journal; v. 30, n. 3, p. 266-271, 2019-06-03.
Abstract

Abstract The aim of this study was to verify whether modifications made in a hard chairside reline resin by an ethyl-cyanoacrylate adhesive, ECA (Super Bonder®, Loctite, Itapevi, SP, Brazil) would be able to inhibit or reduce Candida albicans biofilm formation on its surface, comparing to a commercial surface sealant (BisCover®, Bisco, Schaumburg, USA). Reline resin specimens were fabricated and randomly divided into 6 groups (n=8): CG (control group), no surface treatment; ECA1, ECA coating on the surface before sterilization; ECA2, ECA coating after sterilization; ECA3, ECA incorporated in the resin bulk; DPE1, BisCover® coating before sterilization; DPE2, BisCover® coating after sterilization. Specimens were inoculated with C. albicans SC5314 (1x107 cells/mL) and incubated for 24 h. Then, the biofilm were stained with LIVE/DEAD® BaclightTM L7007 Kit and analyzed by Confocal Laser Scanning Microscopy. The images were evaluated by bioImageL® v.2.0 software and total biovolume (µm3), viable cells (%), and covered area (%) were calculated. Data were statistically analyzed by Kruskal-Wallis and Dunn tests (p<0.05). Results showed that ECA-coated groups presented better results, reducing C. albicans biofilm formation. Acquired images revealed that these groups (ECA1 and ECA2) presented a reduced number of cells, mostly in yeast form (less pathogenic), while the other groups presented higher number of cells, mostly in hyphae form (more pathogenic). Based on these findings, a beneficial effect of Super Bonder® coating reline resins surface could be demonstrated, suggesting a promising way to prevent fungal biofilm formation on dentures. (AU)

FAPESP's process: 14/09426-3 - Effect of tissue sealant based on snake venom and cyanoacrylates applied on acrylic resin's surface on quantity and activity of C. albicans cells, cytotoxicity on gingival fibroblasts (phase I) and surfaces degradation assay (phase II)
Grantee:Vinicíus Carvalho Porto
Support Opportunities: Regular Research Grants